Specifications Table

Product Name – Fluorescent F 254 Indicator for TLC

Quantity/Pack Size – 50 gm

Form – Powder

Grade – Laboratory Grade

Application – Thin Layer Chromatography (TLC) visualization

Product Overview

The Fluorescent F 254 Indicator is an indispensable reagent designed for enhanced visualization in Thin Layer Chromatography (TLC). This high-purity powder acts as a universal indicator, enabling the clear detection of a wide range of organic compounds separated on TLC plates. When incorporated into the stationary phase, the F 254 indicator fluoresces under UV light at 254 nm. Compounds that absorb UV light at this wavelength will quench this fluorescence, appearing as dark spots against a bright fluorescent background. This mechanism provides a highly sensitive and non-destructive method for identifying separated analytes, making it a cornerstone for qualitative and semi-quantitative analysis in various scientific disciplines. Its reliable performance ensures consistent results, aiding researchers and students in achieving accurate chromatographic separations. The fine powder form allows for easy incorporation into adsorbent slurries during plate preparation, guaranteeing uniform distribution and optimal detection capabilities. This indicator is a vital component for ensuring the integrity and clarity of your TLC results, supporting rigorous experimental analysis and discovery.

FAQs

1. What is the primary function of Fluorescent F 254 Indicator in TLC?

The primary function of Fluorescent F 254 Indicator is to enable the visualization of separated compounds on Thin Layer Chromatography plates under ultraviolet light, particularly at 254 nm. It helps in detecting compounds that absorb UV radiation by appearing as dark spots against a fluorescent background.

2. How should Fluorescent F 254 Indicator be prepared or used for optimal results?

Fluorescent F 254 Indicator is typically incorporated into the TLC adsorbent (like silica gel) slurry during the preparation of TLC plates. A small percentage of the indicator is mixed uniformly with the adsorbent before spreading, ensuring an even layer for consistent fluorescence.

3. Is this F 254 Indicator compatible with different types of TLC plates?

Yes, Fluorescent F 254 Indicator is broadly compatible with common TLC plate adsorbents such as silica gel, alumina, and cellulose. Its effectiveness relies on the ability of the underlying stationary phase to support its fluorescence properties.

4. What are the recommended storage conditions for Fluorescent F 254 Indicator?

For optimal shelf life and performance, Fluorescent F 254 Indicator should be stored in a cool, dry place, away from direct sunlight and moisture. Ensure the container is tightly sealed to prevent contamination.

5. Can Fluorescent F 254 Indicator be used for compounds that do not absorb UV light?

Fluorescent F 254 Indicator is specifically designed to visualize compounds that absorb UV light at 254 nm. For compounds that do not absorb UV, alternative visualization methods such as chemical stains or derivatization reagents would be necessary.