Description
Specifications Table
| Product Name | Fmoc-Gln(Trt)-Wang Resin (100-200 Mesh) | 8.56007.0001 | Merck |
|---|---|
| Quantity/Pack Size | 1 G |
| Form | Resin |
| Grade | Research Grade |
| Application | Peptide Synthesis, Solid-Phase Synthesis |
Product Description
When it comes to peptide synthesis, the quality of your resin can make or break your experiment. Fmoc-Gln(Trt)-Wang Resin (100-200 mesh) from Merck is a trusted choice for researchers who need consistent, high-yield results. This resin is pre-loaded with Fmoc-protected glutamine, making it ready for direct use in solid-phase peptide synthesis (SPPS). The 100-200 mesh size ensures optimal solvent flow and reaction efficiency, while the Trt (trityl) protecting group provides stability during synthesis.
Whether you’re working on therapeutic peptides, biochemical studies, or custom peptide projects, this resin delivers reliability. The Wang resin backbone is compatible with standard Fmoc chemistry, allowing seamless integration into your existing protocols. Each batch is rigorously tested to meet Merck’s stringent quality standards, so you can focus on your research without worrying about variability.
For labs in India, eqipped makes it easy to buy Fmoc-Gln(Trt)-Wang Resin online with fast shipping and competitive pricing. No more waiting weeks for imports—get what you need when you need it.
Why This Resin Stands Out
Not all resins are created equal. Here’s what sets this product apart:
The 100-200 mesh size strikes the perfect balance between surface area and solvent accessibility. Too fine, and you risk clogging; too coarse, and coupling efficiency drops. This resin hits the sweet spot for most SPPS applications.
The Trt protecting group on glutamine is crucial for preventing side reactions. Unlike other protecting groups, Trt is stable under acidic conditions but can be easily removed when needed, giving you better control over your synthesis.
Merck’s reputation for consistency means you won’t waste time troubleshooting failed syntheses due to resin quality. Every gram is designed to perform predictably, batch after batch.
If you’re scaling up from small-scale trials to larger productions, this resin adapts seamlessly. Its uniform particle size ensures consistent results whether you’re synthesizing milligrams or grams of peptide.
Applications in Research
This resin isn’t just for routine peptide synthesis. Researchers across India use it for:
Developing therapeutic peptides for drug discovery, where purity and yield are non-negotiable. The high loading capacity of this resin helps maximize output, reducing the cost per peptide.
Studying protein-protein interactions by synthesizing peptide fragments. The clean deprotection profile of Fmoc-Gln(Trt) ensures your peptides fold correctly for accurate binding assays.
Creating custom peptides for enzymatic studies or as standards for mass spectrometry. The resin’s compatibility with automated synthesizers makes it a favorite in high-throughput labs.
Educational settings where students need reliable results for their experiments. Unlike cheaper alternatives, this resin minimizes failures, so students can learn techniques without frustration.
Handling and Storage Tips
To get the most out of your Fmoc-Gln(Trt)-Wang Resin, follow these best practices:
Store the resin in a desiccator at 2-8°C to prevent moisture absorption, which can degrade performance. Always allow it to reach room temperature before opening to avoid condensation.
Use dry solvents like DMF or DCM for swelling and washing. Even trace water can cause premature deprotection or side reactions.
If you’re not using the entire 1g pack at once, divide it into smaller portions in airtight containers. This prevents repeated exposure to humidity.
For automated synthesizers, check the resin’s swelling properties in your specific instrument. Some systems may require slight adjustments to solvent volumes for optimal performance.
Common Challenges and Solutions
Even with high-quality resin, peptide synthesis can be tricky. Here’s how to troubleshoot common issues:
Low coupling efficiency? Try double coupling or increasing the excess of amino acid. The Trt group on glutamine is bulky, so sometimes it needs a little extra push.
Seeing deletion sequences in your final peptide? Ensure your deprotection steps are complete. Fmoc removal should be confirmed with a negative Kaiser test before proceeding.
Resin clumping during synthesis? Swell the resin thoroughly before starting and use gentle agitation. The 100-200 mesh size is less prone to clumping than finer resins, but proper technique still matters.
Unexpected side products? Check your solvent quality. Even “HPLC-grade” solvents can contain impurities that affect sensitive syntheses. Consider distilling DMF before use for critical applications.
Risk Note
This product is for research use only. Not for human or veterinary use. Handle with standard laboratory precautions. Wear appropriate PPE (gloves, goggles, lab coat) when handling. Avoid inhalation and skin contact. Dispose of according to local regulations.
FAQs
What is the loading capacity of this Fmoc-Gln(Trt)-Wang Resin?
The typical loading capacity is 0.5-0.8 mmol/g, but always refer to the certificate of analysis for your specific batch. Merck provides this data to ensure you can calculate reagent quantities accurately.
Can I use this resin for C-terminal amide peptides?
Yes, Wang resin is ideal for synthesizing C-terminal amide peptides. After cleavage, the peptide will have a C-terminal amide, which is common in many bioactive peptides.
How do I confirm the resin is properly swollen before starting synthesis?
Swelling is complete when the resin beads appear transparent and uniform. For 1g of resin, use about 10mL of DMF and let it swell for 30-60 minutes with occasional gentle mixing.
What’s the difference between this and other glutamine-protected resins?
The Trt protecting group is more stable than alternatives like Pbf or Boc under certain conditions. It’s particularly useful when you need orthogonal protection strategies in complex syntheses.
Does eqipped offer bulk discounts for larger quantities?
Yes, for orders above 5g, contact our support team for bulk pricing. We work with research labs and institutions across India to provide cost-effective solutions for large-scale projects.
