Specifications Table

Product Name – FMOC-L-Cys(StBu)-OH (N-Fmoc-S-tert-butylthio-L-cysteine)

Quantity/Pack Size – 1 gm

Form – Crystalline Powder

Grade – Research Grade, >98%

Application – Solid-Phase Peptide Synthesis, Biochemical Research, Protein Chemistry

Product Overview

FMOC-L-Cys(StBu)-OH, chemically known as N-Fmoc-S-tert-butylthio-L-cysteine, stands as a critical and highly specialized building block in the realm of advanced biochemical research, particularly within solid-phase peptide synthesis (SPPS). This meticulously protected amino acid derivative is engineered to facilitate the precise and efficient construction of intricate peptide sequences. The Fluorenylmethyloxycarbonyl (Fmoc) group provides a robust, base-labile protecting strategy for the alpha-amino group, which is fundamental for the stepwise elongation of peptides in SPPS protocols. Simultaneously, the S-tert-butylthio moiety plays an indispensable role by effectively protecting the thiol functionality of L-cysteine. This protection is paramount in preventing undesirable side reactions, such as oxidation or unwanted disulfide bond formation, which can compromise the fidelity of the synthesized peptide chain during the rigorous conditions of synthesis. The presence of this protecting group ensures the integrity of the cysteine residue until its selective deprotection is strategically performed, typically after the full peptide sequence has been assembled. Researchers relying on high-fidelity peptide constructs will find this high-purity compound invaluable. It is essential for those demanding rigorous control over their peptide synthesis, crucial for studying protein structure, investigating biological functions, and pioneering novel therapeutic agents. Our FMOC-L-Cys(StBu)-OH is produced under stringent quality control standards, ensuring exceptional consistency and purity, thereby providing reliable incorporation of cysteine residues into peptides and contributing to the synthesis of stable, biologically active molecules for a diverse spectrum of scientific explorations.

FAQs

1. What is the primary application of FMOC-L-Cys(StBu)-OH?

It is primarily used as a protected amino acid monomer in solid-phase peptide synthesis (SPPS) for incorporating cysteine residues into peptide sequences, crucial for building complex peptides.

2. Why is the S-tert-butylthio protecting group used for cysteine?

The S-tert-butylthio group is employed to protect the highly reactive thiol (sulfhydryl) group of cysteine during peptide synthesis, effectively preventing unwanted side reactions like oxidation or disulfide bond formation until its controlled removal post-synthesis.</

3. What is the recommended storage condition for this compound?

For optimal stability and to maintain its purity, FMOC-L-Cys(StBu)-OH should be stored in a cool, dry place, typically at -20°C, protected from both light and moisture. Ensure the container is always tightly sealed.

4. Is this product suitable for high-throughput peptide synthesis?

Yes, due to its high purity and controlled reactivity, this compound is well-suited for integration into both manual and automated high-throughput solid-phase peptide synthesis workflows, consistently delivering reliable results.

5. What are some common alternatives for protecting cysteine in peptide synthesis?

Other widely used protecting groups for cysteine include S-Trityl (Trt), S-Acetamidomethyl (Acm), and S-tert-Butyl (tBu). Each alternative offers distinct cleavage conditions and properties, allowing researchers to select based on their specific synthesis strategy.