Description
Specifications Table
| Product Name | Hektoen Enteric Agar, w/ 1.4% Agar | M467F-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Salmonella and Shigella in microbiology |
Product Description
Hektoen Enteric Agar is a specialized medium designed for the selective isolation and differentiation of Salmonella and Shigella species from clinical and environmental samples. This agar contains bile salts and dyes that inhibit the growth of Gram-positive bacteria while allowing Gram-negative enteric pathogens to thrive. The presence of lactose and sucrose helps distinguish lactose-fermenting colonies from non-lactose fermenters, aiding in preliminary identification.
This 500gm pack from Himedia is ideal for microbiology labs, research institutions, and educational settings where accurate bacterial isolation is critical. Whether you’re conducting routine diagnostic tests or advanced research, this agar provides reliable results with clear colony differentiation.
For researchers and students working on enteric pathogen studies, Hektoen Enteric Agar is an essential tool. Its formulation ensures consistent performance, making it a trusted choice among microbiologists. If you’re looking to buy Hektoen Enteric Agar online, eqipped offers a seamless purchasing experience with fast delivery across India.
Key Applications
Hektoen Enteric Agar is widely used in:
- Clinical microbiology labs for pathogen identification
- Food and water testing for Salmonella and Shigella contamination
- Educational institutions for microbiology practicals
- Research projects involving enteric bacteria
Why This Agar Stands Out
Unlike generic media, Hektoen Enteric Agar is specifically formulated to suppress unwanted bacterial growth while enhancing the visibility of target pathogens. The inclusion of bromothymol blue and acid fuchsin dyes creates distinct colony colors, making it easier to identify Salmonella (blue-green colonies) and Shigella (colorless colonies). This level of precision is crucial for accurate diagnostics and research.
For those searching for a reliable Hektoen Enteric Agar supplier, eqipped ensures you receive authentic Himedia products with proper documentation. Whether you need it for routine lab work or specialized studies, this agar delivers consistent performance every time.
Usage Guidelines
To prepare the medium, suspend 52 grams of the powder in 1 liter of distilled water. Heat to boiling to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes. Store the prepared plates at 2-8°C and use within the recommended shelf life.
Always follow standard microbiological practices when handling this medium. For best results, inoculate the plates with a sterile loop or swab and incubate at 35-37°C for 18-24 hours. Observe colony morphology and color changes for preliminary identification.
Safety Considerations
While Hektoen Enteric Agar is generally safe for lab use, it contains bile salts and dyes that may cause irritation. Wear appropriate personal protective equipment (PPE) such as gloves and lab coats when handling. Avoid inhalation of the powder and ensure proper ventilation in the workspace.
Dispose of used plates and contaminated materials according to biohazard waste protocols. This product is for professional laboratory use only and should not be ingested or used for non-diagnostic purposes.
FAQs
What is Hektoen Enteric Agar used for?
It is used for the selective isolation and differentiation of Salmonella and Shigella species from clinical and environmental samples.
How do I prepare Hektoen Enteric Agar?
Suspend 52 grams in 1 liter of distilled water, boil to dissolve, autoclave at 121°C for 15 minutes, cool to 45-50°C, and pour into sterile plates.
What do the colony colors indicate?
Salmonella typically appears as blue-green colonies, while Shigella appears colorless. Lactose-fermenting bacteria produce yellow or orange colonies.
Can I buy Hektoen Enteric Agar in smaller quantities?
This product is available in a 500gm pack. For bulk requirements, contact eqipped for customized solutions.
Is this agar suitable for student labs?
Yes, it is commonly used in educational settings for microbiology practicals due to its clear differentiation of enteric pathogens.
