Description
Specifications Table
Product Name – Hematoxylin Powder Indicator
Quantity/Pack Size – 5 g
Form – Powder
Grade – Laboratory Grade
Application – Histological staining, microscopy
Product Overview
Hematoxylin powder is a vital laboratory reagent used for staining cell nuclei in histological preparations. Derived from the heartwood of the logwood tree (*Haematoxylin campechianum*), this natural dye forms a deep blue-purple complex when oxidized to hematein, binding selectively to acidic components like nucleic acids. Its high purity ensures consistent and reliable staining results, making it indispensable for microscopy and cytological studies. The powder form allows for customizable solution preparation, catering to specific staining protocols. With excellent solubility in water and alcohol, it integrates seamlessly into standard staining procedures. This lab-grade hematoxylin is free from impurities, ensuring minimal background interference and sharp contrast in stained samples. Ideal for routine and specialized staining applications, it delivers uniform color intensity and long-term stability when stored properly. Researchers and lab technicians rely on its reproducibility for accurate cellular visualization, supporting precise diagnostic and experimental outcomes.
FAQs
1. What is the solubility of hematoxylin powder in common lab solvents?
Hematoxylin powder is soluble in water, ethanol, and glycerol, but its solubility improves when dissolved in slightly alkaline solutions or after oxidation to hematein.
2. Can this hematoxylin powder be used for progressive and regressive staining techniques?
Yes, this hematoxylin powder is suitable for both progressive staining (direct use without differentiation) and regressive staining (with post-stain differentiation using acid or alcohol).
3. How should hematoxylin powder be stored to maintain its efficacy?
Store the powder in an airtight container, protected from light and moisture, at room temperature (15–25°C). Avoid exposure to oxidizing agents to prevent premature degradation.
4. Is this hematoxylin compatible with eosin for H&E staining?
Absolutely. This hematoxylin pairs well with eosin in H&E (hematoxylin and eosin) staining, providing clear nuclear contrast while eosin stains cytoplasmic components pink.
5. What alternatives exist if hematoxylin staining yields inconsistent results?
If results vary, check pH levels (optimal range: 6.0–7.5) or consider using a mordant like aluminum sulfate. Alternatives include Mayer’s hematoxylin or Gill’s hematoxylin for pre-formulated stability.
