Description
Specifications Table
| Product Name | HEPES Buffer extrapure, 99% | 29768 | SRL Chem |
|---|---|
| Pack Size | 10 Gms, 25 Gms, 100 Gms |
| Form | Powder |
| Grade | Extrapure (99%) |
| Application | Cell culture, biochemical assays, pH buffering |
Product Description
HEPES buffer is a widely used biological buffer in research labs, particularly for cell culture and biochemical applications. This extrapure-grade HEPES buffer from SRL Chem ensures high purity (99%), making it ideal for sensitive experiments where pH stability is critical.
Whether you’re working on mammalian cell culture, protein purification, or enzymatic assays, this HEPES buffer provides consistent performance. Its zwitterionic nature minimizes interference with biological systems, making it a preferred choice over traditional buffers like Tris or phosphate.
At eqipped, we understand the importance of reliable lab supplies. That’s why we source HEPES buffer from trusted manufacturers like SRL Chem, ensuring you get a product that meets stringent quality standards. Whether you need a small 10g pack for occasional use or a 100g bulk quantity for large-scale experiments, we’ve got you covered.
Looking for HEPES buffer for cell culture? Order now and get fast delivery across India.
HEPES buffer is particularly useful in CO₂-independent cell culture systems, where it helps maintain physiological pH without relying on bicarbonate buffering. Researchers also use it in electrophoresis, chromatography, and other molecular biology techniques where precise pH control is essential.
When preparing HEPES buffer solutions, always use high-quality water (preferably Milli-Q or equivalent) and adjust the pH carefully. A common 1M HEPES buffer solution can be prepared by dissolving the appropriate amount in water and adjusting the pH with NaOH or HCl.
Need HEPES buffer for your lab? Shop now at eqipped for authentic SRL Chem products.
FAQs
What is HEPES buffer used for?
HEPES buffer is primarily used in cell culture to maintain stable pH levels, especially in CO₂-independent systems. It’s also used in biochemical assays, protein purification, and molecular biology techniques.
How do I prepare a 1M HEPES buffer solution?
To prepare a 1M HEPES buffer solution, dissolve 238.3g of HEPES powder in 800ml of distilled water. Adjust the pH to the desired value (usually 7.2-7.4) with NaOH or HCl, then bring the volume to 1L with water. Sterilize by filtration if needed.
Is HEPES buffer suitable for all cell types?
HEPES buffer is generally safe for most mammalian cell types. However, some sensitive cells may require specific media formulations. Always check compatibility with your cell line before use.
What is the shelf life of HEPES buffer?
HEPES buffer powder has a long shelf life when stored properly (cool, dry place). Once dissolved, the solution should be stored at 4°C and used within a few weeks to prevent contamination.
Can I buy HEPES buffer in bulk from eqipped?
Yes, eqipped offers HEPES buffer in multiple pack sizes (10g, 25g, 100g) to suit your lab’s needs. Contact us for bulk orders or custom requirements.
