Description
Specifications Table
| Product Name | HEPES Buffer for cell culture, 99.5%, Endotoxin (BET) 0.05EU/mg |
|---|---|
| Quantity/Pack Size | 25 Gms, 100 Gms |
| Form | Powder |
| Grade | Cell Culture Grade |
| Application | Cell culture media, buffer preparation, biochemical assays |
Product Description
When it comes to maintaining stable pH in cell culture experiments, HEPES buffer is a go-to choice for researchers and lab professionals. The HEPES buffer for cell culture from SRL Chem offers 99.5% purity with an ultra-low endotoxin level of 0.05EU/mg, ensuring minimal interference in sensitive biological systems. Whether you’re working on mammalian cell lines, microbial cultures, or enzyme assays, this buffer provides consistent performance.
Unlike traditional bicarbonate buffers, HEPES maintains pH stability even in open-air conditions, making it ideal for CO₂-free incubations. Its zwitterionic nature reduces toxicity, allowing for higher concentrations without compromising cell viability. Researchers across India trust this buffer for applications ranging from protein expression studies to stem cell research.
At eqipped, we understand the importance of reliable lab supplies. That’s why we source this HEPES buffer directly from SRL Chem, ensuring you receive a product that meets international quality standards. The powder form allows for flexible preparation, whether you need small-scale experiments or bulk media formulation.
Need a dependable HEPES buffer for your next cell culture project? Order online today and experience hassle-free delivery across India.
Key Applications
This high-purity HEPES buffer finds extensive use in:
- Mammalian cell culture maintenance
- Preparation of balanced salt solutions
- Protein purification protocols
- Enzyme activity assays
- Cryopreservation media formulation
Why This HEPES Buffer Stands Out
The 99.5% purity level combined with the stringent endotoxin control (0.05EU/mg) makes this product particularly suitable for sensitive applications. Many standard HEPES buffers in the market contain higher endotoxin levels that can trigger unwanted immune responses in cell cultures. This version eliminates that risk, providing cleaner results in your experiments.
For researchers working with primary cell cultures or stem cells, where even minor contaminants can alter outcomes, this buffer offers peace of mind. The consistent batch-to-batch quality from SRL Chem means you can rely on reproducible results in your lab work.
Storage and Handling Recommendations
To maintain the integrity of your HEPES buffer:
- Store the powder at room temperature (15-30°C)
- Keep the container tightly sealed to prevent moisture absorption
- Once prepared in solution, store at 2-8°C for short-term use
- For long-term storage of solutions, aliquot and freeze at -20°C
Safety Considerations
While HEPES is generally considered non-toxic, standard lab safety practices should be followed:
- Wear appropriate PPE (gloves, lab coat) when handling
- Avoid inhalation of powder
- Dispose of waste according to local regulations
FAQs
What is the ideal working concentration for HEPES buffer in cell culture?
Most cell culture applications use HEPES at concentrations between 10-25 mM. For standard DMEM or RPMI media, 20 mM HEPES provides excellent buffering capacity without toxicity.
Can this HEPES buffer be used for bacterial cell cultures?
Yes, this high-purity HEPES buffer works well for bacterial cultures, though some bacterial species may have specific pH requirements that need adjustment.
How does the endotoxin level of 0.05EU/mg benefit my experiments?
Lower endotoxin levels reduce the risk of activating immune responses in cell cultures, which is crucial for studies involving immune cells or when working with sensitive cell lines.
What’s the shelf life of this HEPES buffer powder?
When stored properly in its original packaging, the powder remains stable for at least 2 years from the date of manufacture.
Can I buy HEPES buffer in smaller quantities for trial purposes?
Yes, eqipped offers this HEPES buffer in 25g packs, perfect for small-scale experiments or testing before larger purchases.
