High-purity HEPES free acid (99%+) ensures reliable buffering in molecular biology. DNase, RNase, and protease-free for contamination-sensitive workflows.
Price range: ₹8,948.00 through ₹51,467.00
Product Name – HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free
Quantity/Pack Size – 50GR, 500GR
Form – Free acid (powder)
Grade – Molecular biology grade (99%+ purity)
Application – pH buffering in cell culture, nucleic acid research, and enzymatic assays
HEPES free acid is a zwitterionic organic buffering agent designed for maintaining physiological pH in biological systems. With a purity of 99%+, this molecular biology-grade reagent is rigorously tested to be free from DNase, RNase, and protease contaminants, ensuring integrity in sensitive applications. Its pKa of 7.5 at 25°C makes it ideal for buffering in the 6.8–8.2 range, providing stable pH control without interfering with metal ions or biological processes. The powder form allows for flexible preparation of solutions at desired concentrations, while its low toxicity and minimal cellular effects enhance compatibility with live-cell experiments. Unlike bicarbonate-based buffers, HEPES does not produce CO₂, eliminating pH fluctuations in open systems. This high-quality free acid variant avoids sodium or potassium counterions, offering precise control over ionic composition in experimental setups. Its chemical stability under standard storage conditions ensures long shelf life without degradation.
HEPES free acid is highly soluble in water, with a solubility of approximately 238 mg/mL at 20°C. For complete dissolution, adjust the pH to 7.0–7.6 using NaOH or KOH.
Yes, HEPES solutions (typically 10–50 mM) can be autoclaved at 121°C for 20 minutes without significant degradation or pH shifts.
HEPES provides more stable pH control in atmospheric CO₂ conditions compared to Tris, which is highly temperature-sensitive. HEPES also exhibits lower toxicity at equivalent concentrations.
Store the powder at room temperature (15–25°C) in a tightly sealed container, protected from moisture and light. Prepared solutions should be refrigerated at 2–8°C.
Yes, HEPES does not chelate divalent cations like EDTA, making it fully compatible with media containing calcium or magnesium salts.
| Size | 50GR, 500GR |
|---|
Albumin Fixative Mayer-Lancer, high-grade for lab applications. Ideal for fixing tissues.
High-purity Aluminium Fluoride suitable for various lab applications.
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Ammonium Borate, high purity grade, ideal for lab research and education. Essential for various lab applications.
Ammonium Hydrogen Orthophosphate (Dibasic) – High purity, suitable for laboratory use. Essential for various chemical applications.
Price range: ₹8,948.00 through ₹51,467.00
High-purity HEPES free acid (99%+) ensures reliable buffering in molecular biology. DNase, RNase, and protease-free for contamination-sensitive workflows.
Product Name – HEPES free acid, 99+%, for molecular biology, DNase, Rnase and Protease free
Quantity/Pack Size – 50GR, 500GR
Form – Free acid (powder)
Grade – Molecular biology grade (99%+ purity)
Application – pH buffering in cell culture, nucleic acid research, and enzymatic assays
HEPES free acid is a zwitterionic organic buffering agent designed for maintaining physiological pH in biological systems. With a purity of 99%+, this molecular biology-grade reagent is rigorously tested to be free from DNase, RNase, and protease contaminants, ensuring integrity in sensitive applications. Its pKa of 7.5 at 25°C makes it ideal for buffering in the 6.8–8.2 range, providing stable pH control without interfering with metal ions or biological processes. The powder form allows for flexible preparation of solutions at desired concentrations, while its low toxicity and minimal cellular effects enhance compatibility with live-cell experiments. Unlike bicarbonate-based buffers, HEPES does not produce CO₂, eliminating pH fluctuations in open systems. This high-quality free acid variant avoids sodium or potassium counterions, offering precise control over ionic composition in experimental setups. Its chemical stability under standard storage conditions ensures long shelf life without degradation.
HEPES free acid is highly soluble in water, with a solubility of approximately 238 mg/mL at 20°C. For complete dissolution, adjust the pH to 7.0–7.6 using NaOH or KOH.
Yes, HEPES solutions (typically 10–50 mM) can be autoclaved at 121°C for 20 minutes without significant degradation or pH shifts.
HEPES provides more stable pH control in atmospheric CO₂ conditions compared to Tris, which is highly temperature-sensitive. HEPES also exhibits lower toxicity at equivalent concentrations.
Store the powder at room temperature (15–25°C) in a tightly sealed container, protected from moisture and light. Prepared solutions should be refrigerated at 2–8°C.
Yes, HEPES does not chelate divalent cations like EDTA, making it fully compatible with media containing calcium or magnesium salts.
| Size | 50GR, 500GR |
|---|
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Alpha Naphthol, high purity, ideal for various lab applications. Suitable for students, researchers, and educators.
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