Description
Specifications Table
Product Name – HEPES sodium salt, 99%
Quantity/Pack Size – 1KG, 25GR, 100GR, 500GR
Form – Powder
Grade – 99%
Application – pH buffering agent, cell culture, biochemical assays
Product Overview
HEPES sodium salt (99% purity) is a highly effective zwitterionic buffering agent widely used in biological and biochemical research. Its exceptional pH stability (6.8–8.2) makes it indispensable for maintaining physiological conditions in cell culture media, enzyme assays, and protein purification processes. Unlike traditional buffers, HEPES exhibits minimal interference with biological systems, ensuring accurate experimental outcomes. The sodium salt form enhances solubility in aqueous solutions, eliminating precipitation risks even at higher concentrations. This grade is rigorously tested to meet stringent quality standards, guaranteeing low endotoxin and heavy metal contamination levels. Researchers rely on its consistent performance across temperature variations, making it a preferred choice for sensitive applications like mammalian cell culture and molecular biology protocols. The powder form allows for flexible preparation of stock solutions, while its non-toxic profile ensures compatibility with live-cell experiments. Whether used in basic research or advanced biotechnological applications, this HEPES sodium salt delivers reliable buffering capacity without compromising cellular viability or experimental integrity.
FAQs
1. What is the optimal pH range for HEPES sodium salt?
HEPES sodium salt maintains effective buffering between pH 6.8 and 8.2, with maximum capacity around pH 7.5, making it ideal for physiological studies.
2. Can this HEPES salt be used for DNA/RNA experiments?
Yes, the 99% pure grade is suitable for nucleic acid work as it lacks DNase/RNase activity and doesn’t interfere with hybridization reactions.
3. How does the sodium salt form differ from free acid HEPES?
The sodium salt offers improved water solubility and eliminates the need for pH adjustment with NaOH, providing more consistent buffering performance.
4. What storage conditions are recommended for long-term stability?
Store in a cool, dry place at room temperature (15–25°C) in tightly sealed containers to prevent moisture absorption and degradation.
5. Is this HEPES salt compatible with serum-free cell culture media?
Absolutely, its high purity and low endotoxin levels make it particularly suitable for serum-free and chemically defined media formulations.
