Description
Specifications Table
Product Name – HEPES sodium salt, 99+%, for molecular biology, DNAse, RNAse and Protease free
Quantity/Pack Size – 100GR, 500GR
Form – Powder
Grade – Molecular Biology Grade
Application – Buffering agent, cell culture, nucleic acid research
Product Overview
HEPES sodium salt (99%+ purity) is a high-performance buffering agent specifically designed for molecular biology applications where enzymatic contamination is a concern. This ultra-pure grade is rigorously tested to ensure it is free from DNAse, RNAse, and protease activity, making it ideal for sensitive biochemical assays, cell culture media, and nucleic acid research. The zwitterionic nature of HEPES provides excellent pH stability (pKa 7.5 at 25°C) across a wide temperature range, minimizing pH shifts that could compromise experimental results. Its low toxicity and high solubility in water (up to 1.5 M) allow for versatile use in various biological systems without interfering with cellular processes. The sodium salt form enhances solubility and compatibility with physiological solutions, ensuring consistent performance in critical applications. Each batch undergoes strict quality control to guarantee lot-to-lot consistency, meeting the stringent requirements of advanced research laboratories.
FAQs
1. What is the pH range where HEPES sodium salt is most effective?
HEPES sodium salt maintains optimal buffering capacity between pH 6.8 and 8.2, with a pKa of 7.5 at 25°C, making it particularly useful for physiological pH applications.
2. Can this HEPES sodium salt be used for mammalian cell culture?
Yes, this grade is specifically formulated to be non-toxic and compatible with mammalian cell culture systems, supporting cell viability and growth.
3. How should HEPES sodium salt be stored to maintain its purity?
Store in a cool, dry place at room temperature (15-30°C) in a tightly sealed container to prevent moisture absorption and contamination.
4. Is this product compatible with common molecular biology enzymes?
The DNAse, RNAse, and protease-free certification ensures compatibility with sensitive enzymes used in PCR, cloning, and other molecular biology techniques.
5. What alternatives exist if HEPES sodium salt is unavailable?
Alternatives like MOPS or TAPS can be considered, though their pKa values differ (MOPS: 7.2, TAPS: 8.4), which may require protocol adjustments.
