Description
Specifications Table
Product Name – Hexokinase from Ex- Saccharomyces sp. 150 U/mg
Quantity/Pack Size – 5000 units
Form – Lyophilized Powder
Grade – Biochemical Grade
Application – Glucose Metabolism Studies, Glycolysis Research, ATP-dependent Phosphorylation
Product Overview
Hexokinase (EC 2.7.1.1) is a crucial enzyme that initiates glucose metabolism by catalyzing the phosphorylation of glucose into glucose-6-phosphate. This fundamental reaction is the initial committed step of glycolysis, a central pathway in cellular energy production. Our Hexokinase, meticulously isolated from *Saccharomyces sp.* (yeast), boasts a high specific activity of 150 U/mg. This ensures exceptional performance and consistency in a wide array of biochemical applications, making it an indispensable reagent for demanding laboratory environments focused on biochemistry, molecular biology, and metabolic research. The enzyme undergoes stringent purification to meet rigorous quality standards, guaranteeing its reliability for sensitive assays.
Its pivotal role in glucose phosphorylation makes it essential for scientists investigating cellular energetics, carbohydrate metabolic pathways, and enzyme kinetics. The high purity and verified activity level assure its suitability for precise experimental setups, including those involving ATP turnover studies and substrate specificity analyses. Researchers and educators can depend on this Hexokinase to deliver accurate and reproducible experimental outcomes. It is manufactured to maintain optimal stability and enzymatic functionality, providing a dependable solution for complex enzymatic assays. Understanding the intricate mechanisms of life sciences often starts with the precise actions of enzymes like Hexokinase, making it a foundational component for advancing scientific understanding. This product is intended for general laboratory use, supporting a broad spectrum of research endeavors.
FAQs
1. What is the primary function of Hexokinase?
Hexokinase catalyzes the initial phosphorylation of glucose to glucose-6-phosphate, marking the first step in glycolysis and a vital reaction in carbohydrate metabolism.
2. From which organism is this Hexokinase derived?
This Hexokinase is sourced from *Saccharomyces sp.*, commonly known as baker’s yeast, a widely used and well-characterized biological source.
3. What are the recommended storage conditions for this enzyme?
For optimal stability and enzymatic activity, Hexokinase should be stored frozen at -20°C. It is also advisable to protect it from exposure to light and moisture.
4. Can this Hexokinase be used for enzyme kinetic studies?
Yes, given its high specific activity of 150 U/mg and its purified biochemical grade, this Hexokinase is very suitable for detailed enzyme kinetic assays and mechanistic studies.
5. How does the activity of this Hexokinase compare to other commercially available enzymes?
Hexokinase from *Saccharomyces sp.* is a highly characterized and commonly utilized enzyme, renowned for its robust activity and consistent performance across diverse biochemical applications, offering reliable results for researchers.
