Description
Specifications Table
| Product Name | HiCrome® MacConkey Sorbitol Agar Base |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | E. coli detection, microbiology research |
Product Description
HiCrome® MacConkey Sorbitol Agar Base is a specialized medium designed for the selective isolation and differentiation of Escherichia coli, particularly sorbitol-non-fermenting strains like E. coli O157:H7. This agar base is widely used in clinical and research laboratories for its reliability in detecting pathogenic E. coli, making it an essential tool for food safety testing, water analysis, and microbiological studies.
The medium contains sorbitol as the primary carbohydrate source, allowing for the differentiation of sorbitol-fermenting and non-fermenting bacteria. The addition of bile salts and crystal violet inhibits the growth of Gram-positive bacteria, ensuring selective isolation of Gram-negative organisms. The presence of neutral red as a pH indicator helps in visualizing fermentation patterns, with sorbitol-fermenting colonies appearing pink and non-fermenting colonies remaining colorless.
HiCrome® MacConkey Sorbitol Agar Base is easy to prepare and provides consistent results, making it a preferred choice for researchers and lab technicians. Whether you’re conducting routine microbial analysis or advanced research, this agar base offers the precision and reliability needed for accurate E. coli detection.
For those looking to buy MacConkey Sorbitol Agar online, eqipped provides a seamless purchasing experience with authentic Himedia products. Our platform ensures you receive high-quality lab supplies delivered to your doorstep, so you can focus on your research without worrying about procurement.
How to Use HiCrome® MacConkey Sorbitol Agar Base
Preparing and using this agar base is straightforward. Follow these steps for optimal results:
- Dissolve 50 grams of the powder in 1 liter of distilled water.
- Heat the solution to boiling while stirring to ensure complete dissolution.
- Autoclave the medium at 121°C for 15 minutes to sterilize.
- Cool the medium to 45-50°C and pour into sterile Petri dishes.
- Allow the agar to solidify before inoculating with the sample.
- Incubate the plates at 35-37°C for 18-24 hours and observe for colony characteristics.
Sorbitol-fermenting colonies will appear pink, while non-fermenting colonies, such as E. coli O157:H7, will remain colorless. This clear differentiation makes it easier to identify and isolate specific strains for further analysis.
Applications of MacConkey Sorbitol Agar
This agar base is versatile and finds applications in various fields, including:
- Clinical microbiology for detecting pathogenic E. coli in patient samples.
- Food safety testing to identify contamination in meat, dairy, and processed foods.
- Water quality analysis to ensure microbial safety in drinking and recreational water.
- Research laboratories studying bacterial metabolism and antibiotic resistance.
Its selectivity and differential capabilities make it an invaluable tool for professionals working in public health, environmental science, and biomedical research.
Why This Product Stands Out
HiCrome® MacConkey Sorbitol Agar Base from Himedia is trusted by labs across India for its consistency and accuracy. The high-quality formulation ensures minimal batch-to-batch variation, providing reliable results every time. Additionally, the product is designed to meet international standards, making it suitable for both routine testing and advanced research applications.
When you buy MacConkey Sorbitol Agar online from eqipped, you’re choosing a platform that prioritizes authenticity and customer satisfaction. We source directly from reputable manufacturers like Himedia, ensuring that you receive genuine products that meet your lab’s requirements.
Storage and Handling
To maintain the integrity of the product, store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable timeframe to prevent dehydration or contamination. Always follow standard laboratory safety protocols when handling microbial media.
Risk Note
This product is for laboratory use only and should be handled by trained professionals. Avoid inhalation or direct contact with skin and eyes. In case of accidental exposure, rinse thoroughly with water and seek medical advice if necessary. Dispose of used media and contaminated materials according to biohazard waste regulations.
FAQs
What is MacConkey Sorbitol Agar used for?
MacConkey Sorbitol Agar is used for the selective isolation and differentiation of E. coli, particularly sorbitol-non-fermenting strains like E. coli O157:H7. It is commonly used in clinical, food, and water microbiology.
How do I prepare HiCrome® MacConkey Sorbitol Agar Base?
Dissolve 50 grams of the powder in 1 liter of distilled water, heat to boiling, autoclave at 121°C for 15 minutes, cool to 45-50°C, and pour into sterile Petri dishes.
What do the colony colors indicate on this agar?
Pink colonies indicate sorbitol fermentation, while colorless colonies suggest non-fermentation, typical of pathogenic strains like E. coli O157:H7.
Can I buy MacConkey Sorbitol Agar online in India?
Yes, you can buy HiCrome® MacConkey Sorbitol Agar Base online from eqipped, ensuring authentic Himedia products with fast delivery.
What is the shelf life of this product?
The powder has a shelf life of 2-3 years when stored properly in a cool, dry place. Prepared agar plates should be used within a few weeks for best results.
