Description
Specifications Table
| Product Name | HiGlutaXL™ Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) |
|---|---|
| Quantity/Pack Size | 2 × 500 ml |
| Form | Liquid |
| Grade | Cell Culture Grade |
| Application | Cell Culture, Research, Education |
Product Description
HiGlutaXL™ Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F12, 1:1 Mixture) is a versatile and high-performance medium designed to support a wide range of cell types in culture. This 1:1 mixture of DMEM and Ham’s F-12 provides a balanced nutrient environment that promotes optimal cell growth and differentiation.
Why Choose HiGlutaXL™ DMEM F12?
HiGlutaXL™ DMEM F12 is formulated with a unique blend of essential amino acids, vitamins, and inorganic salts, ensuring that your cells receive the nutrients they need to thrive. This medium is particularly suitable for primary cells, stem cells, and other fastidious cell types that require a more complex nutrient mix.
Key Features of HiGlutaXL™ DMEM F12:
1. Balanced Nutrient Mix: The 1:1 mixture of DMEM and Ham’s F-12 provides a comprehensive nutrient profile that supports a wide range of cell types.
2. High Glutamine Content: HiGlutaXL™ DMEM F12 is enriched with L-glutamine, a critical amino acid for cell growth and metabolism.
3. Low Osmolality: The medium is formulated to maintain low osmolality, which is crucial for the health and viability of cultured cells.
4. Quality Assurance: Manufactured under stringent quality control measures to ensure consistency and reliability.
Applications of HiGlutaXL™ DMEM F12:
HiGlutaXL™ DMEM F12 is widely used in various research and educational settings, including:
1. Cell Culture: Ideal for maintaining and growing a variety of cell types, including primary cells, stem cells, and immortalized cell lines.
2. Research: Suitable for in vitro studies, drug screening, and other research applications.
3. Education: Perfect for teaching labs and educational institutions to demonstrate cell culture techniques.
How to Use HiGlutaXL™ DMEM F12:
To ensure optimal performance, follow these guidelines:
1. Storage: Store the medium at 2-8°C. Avoid freezing.
2. Supplementation: Depending on your specific application, you may need to supplement the medium with additional growth factors, serum, or other components.
3. Sterility: Ensure that all handling and culturing procedures are performed under sterile conditions to prevent contamination.
Safety Note:
HiGlutaXL™ DMEM F12 is a high-risk chemical product. Handle with care and follow all safety guidelines. Use appropriate personal protective equipment (PPE) and work in a well-ventilated area.
FAQs
What is the shelf life of HiGlutaXL™ DMEM F12?
The shelf life of HiGlutaXL™ DMEM F12 is typically 12 months from the date of manufacture when stored at 2-8°C.
Can HiGlutaXL™ DMEM F12 be used for primary cell culture?
Yes, HiGlutaXL™ DMEM F12 is suitable for primary cell culture. Its balanced nutrient mix supports the growth and viability of primary cells.
What is the osmolality of HiGlutaXL™ DMEM F12?
The osmolality of HiGlutaXL™ DMEM F12 is designed to be low, typically around 290-310 mOsm/kg, which is optimal for cell culture.
How should HiGlutaXL™ DMEM F12 be stored?
HiGlutaXL™ DMEM F12 should be stored at 2-8°C. Avoid freezing, as this can affect the performance of the medium.
What are the key components of HiGlutaXL™ DMEM F12?
HiGlutaXL™ DMEM F12 contains essential amino acids, vitamins, inorganic salts, and is enriched with L-glutamine, providing a comprehensive nutrient profile for cell culture.
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