Description
Specifications Table
| Product Name | HiPurA™ Plasmid DNA Miniprep Purification Kit |
|---|---|
| Pack Size | 50 preps |
| Form | Kit (columns, buffers, reagents) |
| Grade | Molecular Biology Grade |
| Application | Plasmid DNA purification from bacterial cultures |
Product Description
The HiPurA™ Plasmid DNA Miniprep Purification Kit is a spin column-based DNA purification kit designed for the rapid isolation of high-quality plasmid DNA from bacterial cultures. Utilizing silica membrane technology, the kit enables efficient binding, washing, and elution of plasmid DNA, producing purified DNA suitable for a wide range of molecular biology applications.
This kit is suitable for biotechnology laboratories, molecular biology research facilities, universities, pharmaceutical laboratories, and educational institutions. It is commonly used during recombinant DNA research, plasmid cloning, vector verification, and routine plasmid purification workflows. The purified DNA is compatible with downstream applications such as PCR, DNA sequencing, restriction enzyme digestion, ligation, cloning, and library screening. Typical DNA yields are up to 20 µg from 1–5 ml of bacterial culture, depending on the plasmid and host strain.
The kit contains 50 purification preps and features HiMedia’s HiElute spin columns, allowing rapid processing of multiple samples with reliable DNA recovery and reproducible results. The simple three-step workflow—DNA binding, contaminant removal, and elution—reduces processing time while delivering high-quality plasmid DNA for research applications.
This product is intended exclusively for laboratory, molecular biology, educational, and research use. It is not intended for diagnostic, therapeutic, veterinary, or household applications.
eqipped supplies genuine HiMedia molecular biology kits with secure packaging, reliable delivery across India, and bulk purchasing options for research laboratories, biotechnology companies, universities, and academic institutions.
FAQs
What is the typical yield from this plasmid DNA miniprep kit?
The yield varies based on the bacterial strain and culture conditions, but you can expect 10-30 µg of high-purity plasmid DNA per prep.
Can this kit be used for low-copy plasmids?
Yes, the HiPurA™ kit is optimized for both high-copy and low-copy plasmids, though yields may be lower for the latter.
Is this kit compatible with automated liquid handlers?
While designed for manual use, the spin columns can be adapted for some automated systems with proper validation.
How should I store the kit components?
Store the columns and buffers at room temperature (15-25°C). Avoid freezing or exposure to direct sunlight.
Where can I buy this plasmid DNA miniprep kit in India?
You can buy the HiPurA™ Plasmid DNA Miniprep Purification Kit online from eqipped, a trusted supplier of lab reagents and kits across India.
