Description
Specifications Table
| Product Name | Hygromycin B (HGR) for tissue culture, 90% | 11056 | SRL Chem |
|---|---|
| Quantity/Pack Size | 100 Mg, 250 Mg |
| Form | Powder |
| Grade | Tissue Culture |
| Application | Selection marker in plant and animal cell cultures |
Product Description
Hygromycin B is a widely used aminoglycoside antibiotic derived from Streptomyces hygroscopicus. With 90% purity, this grade is specifically optimized for tissue culture applications, making it an essential tool for researchers working with plant and animal cell lines. Its mechanism of action involves inhibiting protein synthesis by binding to ribosomal RNA, ensuring effective selection of resistant cell lines.
Whether you’re conducting gene transfer experiments or maintaining stable cell lines, Hygromycin B provides reliable selection pressure. The high purity of this product minimizes off-target effects, ensuring consistent results in your experiments. Researchers across India trust eqipped for authentic lab supplies, and this Hygromycin B variant is no exception.
For those looking to buy Hygromycin B online, eqipped offers a seamless purchasing experience with verified quality. The product is available in convenient pack sizes of 100 Mg and 250 Mg, catering to both small-scale and bulk requirements. Always store the powder in a cool, dry place to maintain stability.
Need Hygromycin B for your next tissue culture experiment? Order now and ensure precise selection in your cell lines.
FAQs
What is the working concentration of Hygromycin B in tissue culture?
The typical working concentration ranges from 50 to 400 µg/mL, depending on the cell type. For plant cells, 20-50 µg/mL is common, while animal cells may require 100-400 µg/mL. Always perform a kill curve assay to determine the optimal dose for your specific cell line.
Can Hygromycin B be used for bacterial selection?
No, Hygromycin B is ineffective against most bacteria. It is specifically designed for eukaryotic cells, including plant, animal, and fungal systems. For bacterial selection, consider alternatives like ampicillin or kanamycin.
How should I prepare a stock solution of Hygromycin B?
Dissolve the powder in sterile water or PBS to create a 50 mg/mL stock solution. Filter sterilize using a 0.22 µm syringe filter and store aliquots at -20°C. Avoid repeated freeze-thaw cycles to maintain potency.
Is this product suitable for CRISPR-Cas9 experiments?
Yes, Hygromycin B is commonly used in CRISPR workflows where the hph gene is included as a selection marker. It helps isolate successfully edited cells by eliminating non-resistant populations.
Where can I buy Hygromycin B online in India?
You can purchase authentic Hygromycin B for tissue culture from eqipped. We ensure timely delivery and provide products sourced from trusted manufacturers like SRL Chem.
