Description
Specifications Table
| Product Name | Hygromycin B (HGR Solution), 50mg/ml in Aq. Solution |
|---|---|
| Quantity/Pack Size | 20 ml, 100 ml |
| Form | Aqueous Solution |
| Grade | Lab Grade |
| Application | Cell culture selection, antibiotic resistance studies |
Product Description
Hygromycin B is a widely used aminoglycoside antibiotic derived from Streptomyces hygroscopicus, primarily employed in molecular biology and cell culture applications. This 50mg/ml aqueous solution is a ready-to-use reagent for selecting hygromycin-resistant cells in bacterial, yeast, and mammalian cell cultures. Its broad-spectrum activity makes it an essential tool for genetic engineering, gene expression studies, and stable cell line development.
At eqipped, we ensure this solution meets rigorous quality standards, providing researchers and educators with a reliable selection reagent. Whether you’re working on plasmid maintenance, gene knockout experiments, or protein expression studies, this hygromycin B solution delivers consistent performance.
Need hygromycin B for your next experiment? Order now for fast, pan-India delivery.
Key Applications
Hygromycin B solution is versatile in laboratory settings. It is commonly used for:
- Selecting hygromycin-resistant bacterial and mammalian cells
- Maintaining plasmids with hygromycin resistance markers
- Studying antibiotic resistance mechanisms
- Developing stable cell lines for protein production
Why Hygromycin B?
Unlike other antibiotics, hygromycin B offers several advantages in cell culture:
- High solubility in aqueous solutions
- Effective at low concentrations (typically 50-400 µg/ml)
- Stable under standard lab conditions
- Compatible with most cell culture media
For researchers in India, eqipped provides this solution in convenient pack sizes (20 ml and 100 ml) to suit different experimental needs.
Safety and Handling
Hygromycin B is a potent antibiotic and should be handled with care. Always wear appropriate personal protective equipment (PPE) when working with this solution. Store at 2-8°C and avoid repeated freeze-thaw cycles to maintain stability.
FAQs
What is the working concentration of hygromycin B for mammalian cells?
Typical working concentrations range from 50-400 µg/ml, depending on the cell type. Always perform a kill curve assay to determine the optimal concentration for your specific cells.
Can hygromycin B be used for bacterial selection?
Yes, hygromycin B is effective against both Gram-positive and Gram-negative bacteria, making it suitable for bacterial selection experiments.
How should I store hygromycin B solution?
Store the solution at 2-8°C. For long-term storage, aliquot and freeze at -20°C. Avoid repeated freeze-thaw cycles.
Is this solution filter-sterilized?
Yes, this hygromycin B solution is filter-sterilized and ready for direct use in cell culture applications.
Where can I buy hygromycin B solution online in India?
You can purchase hygromycin B solution from eqipped, a trusted supplier of lab reagents across India. Order now for quick delivery.
