Description
Specifications Table
Product Name – Hygromycin-B
Quantity/Pack Size – 100 mg, 1 gm
Form – Powder
Grade – Laboratory Reagent
Application – Selection Agent in Molecular Biology
Product Overview
Hygromycin-B is a potent aminocyclitol antibiotic derived from *Streptomyces hygroscopicus*. It is widely used as a selective agent in molecular biology to maintain transformed cells containing resistance genes. This high-purity reagent ensures reliable performance in research applications, including bacterial and eukaryotic cell culture. Its broad-spectrum activity makes it essential for selecting genetically modified organisms. Manufactured under stringent quality control, Hygromycin-B from eqipped guarantees consistency and accuracy for laboratory use. Researchers and educators rely on its effectiveness for cloning, transfection, and gene expression studies.
FAQs
1. What is the primary use of Hygromycin-B in labs?
Hygromycin-B is primarily used as a selection agent to identify and maintain cells that have successfully incorporated resistance genes in genetic engineering experiments.
2. Is Hygromycin-B compatible with mammalian cell cultures?
Yes, Hygromycin-B is commonly used in mammalian cell culture systems to select cells expressing the hygromycin resistance gene.
3. Can Hygromycin-B be used in bacterial transformation experiments?
Yes, it is effective in bacterial transformation for selecting transformed colonies carrying the hygromycin resistance marker.
4. How should Hygromycin-B be stored to maintain stability?
Store Hygromycin-B at -20°C in a dry, dark environment to prevent degradation and ensure long-term stability.
5. What are the alternatives to Hygromycin-B for selection?
Alternatives include geneticin (G418), puromycin, and blasticidin, depending on the resistance gene used in the experiment.
