Description
Specifications Table
| Product Name | Quantity/Pack Size | Form | Grade | Application |
|---|---|---|---|---|
| Hygromycin-B | 117995 | CDH | 100mg | Powder | Molecular Biology Grade | Cell culture selection, antibiotic resistance studies |
Product Description
Hygromycin-B is a powerful aminoglycoside antibiotic widely used in molecular biology and cell culture applications. Derived from Streptomyces hygroscopicus, it selectively inhibits protein synthesis in prokaryotic and eukaryotic cells, making it an essential tool for genetic selection. Whether you’re working on stable cell line development or plasmid selection, this high-purity Hygromycin-B ensures consistent and reliable results.
Researchers and students across India trust Hygromycin-B for its effectiveness in maintaining selective pressure in cell cultures. The molecular biology grade ensures minimal interference with experimental outcomes, making it ideal for sensitive applications. If you’re looking to buy Hygromycin-B online, eqipped offers lab-grade quality with fast shipping to support your research needs.
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Hygromycin-B works by binding to the 30S ribosomal subunit, disrupting translation and leading to cell death in susceptible organisms. Its broad-spectrum activity makes it useful in both bacterial and mammalian cell cultures. For best results, follow standard protocols for concentration and incubation times. Always store Hygromycin-B in a dry, cool place to maintain its potency.
Students and educators will find this antibiotic particularly useful for teaching molecular biology techniques. Its straightforward application and clear selection markers make it a staple in lab courses. When you buy Hygromycin-B from eqipped, you’re getting a product that meets international quality standards, ensuring your experiments run smoothly.
Applications of Hygromycin-B
This versatile antibiotic finds use in multiple laboratory applications:
In cell culture, Hygromycin-B serves as a selection agent for cells transfected with hygromycin resistance genes. It’s commonly used in the generation of stable cell lines expressing recombinant proteins or shRNAs. Researchers working on CRISPR-Cas9 experiments also rely on Hygromycin-B for selecting successfully edited cells.
For molecular cloning, Hygromycin-B helps identify bacterial colonies that have taken up plasmids containing the hygromycin resistance gene. This makes it easier to isolate clones with your gene of interest. The antibiotic’s stability in culture media ensures long-lasting selection pressure throughout your experiment.
In plant tissue culture, Hygromycin-B assists in selecting transformed plant cells. This application is particularly valuable in agricultural biotechnology research where genetic modification of crops is studied. The antibiotic’s effectiveness across different biological systems makes it a valuable tool in diverse research settings.
Handling and Storage
To maintain the efficacy of Hygromycin-B, proper handling and storage are crucial. Always wear appropriate personal protective equipment when working with this antibiotic. Store the powder at 2-8°C in a tightly sealed container, protected from light and moisture. For long-term storage, keeping it at -20°C is recommended.
When preparing stock solutions, dissolve Hygromycin-B in sterile water or appropriate buffer. Filter sterilization is recommended for solutions that will be stored for extended periods. Working solutions should be prepared fresh for each experiment to ensure optimal performance. Remember that repeated freeze-thaw cycles can reduce the antibiotic’s effectiveness.
Safety Considerations
While Hygromycin-B is generally safe for laboratory use, proper precautions should be taken. Avoid inhalation or direct contact with skin and eyes. In case of accidental exposure, rinse thoroughly with water. Dispose of waste solutions according to your institution’s biohazard waste protocols.
FAQs
What is the working concentration of Hygromycin-B for mammalian cells?
The typical working concentration ranges from 50 to 400 µg/mL, depending on the cell type. For most cell lines, 200 µg/mL is commonly used. Always perform a kill curve to determine the optimal concentration for your specific cells.
Can Hygromycin-B be used for bacterial selection?
Yes, Hygromycin-B is effective against many bacteria. For E. coli, concentrations between 50 to 100 µg/mL are typically used. The antibiotic works well with common cloning vectors that carry the hygromycin resistance gene.
How stable is Hygromycin-B in culture media?
Hygromycin-B remains stable in culture media at 37°C for at least 2 weeks. For longer experiments, it’s recommended to replenish the antibiotic every 3-4 days to maintain effective selection pressure.
What’s the difference between Hygromycin-B and other selection antibiotics?
Unlike antibiotics like ampicillin or kanamycin, Hygromycin-B works effectively in both prokaryotic and eukaryotic systems. It also has a different mode of action, making it useful for dual selection strategies in complex experiments.
Where can I buy authentic Hygromycin-B online in India?
You can purchase high-quality Hygromycin-B from eqipped, a trusted supplier of lab reagents across India. We offer genuine products with proper documentation and fast delivery to support your research needs.
