Specifications Table
Product Name – IMIDAZOLE 99.9% Molecular Biology
Quantity/Pack Size – 25 g, 100 g
Form – Crystalline Powder
Grade – Molecular Biology
Application – Protein Purification, Buffer Preparation, Crystallization
Product Overview
Imidazole 99.9% Molecular Biology grade is a high-purity crystalline powder designed for critical laboratory applications requiring precision and reliability. This reagent is widely recognized for its role in protein purification, particularly in affinity chromatography, where it effectively elutes histidine-tagged proteins from nickel or cobalt resin columns. Its exceptional solubility in water and organic solvents makes it a versatile choice for buffer preparation, ensuring stable pH conditions in biochemical assays. The molecular biology grade ensures minimal impurities, reducing the risk of contamination in sensitive experiments such as protein crystallization or enzymatic reactions. With a molecular weight of 68.08 g/mol and a melting point of 88-91°C, this compound offers consistent performance across a range of temperatures and conditions. Whether used in small-scale research or large-scale bioprocessing, this Imidazole variant delivers reproducibility and accuracy, making it indispensable in modern molecular biology workflows. Its stability under standard storage conditions further enhances its utility, providing long-term reliability for laboratories demanding high-quality reagents.
FAQs
1. What is the solubility of Imidazole 99.9% in water?
Imidazole 99.9% is highly soluble in water, with a solubility of approximately 633 g/L at 20°C, making it suitable for aqueous buffer systems.
2. Can this Imidazole grade be used for protein crystallization?
Yes, the high purity of this molecular biology grade Imidazole makes it ideal for protein crystallization applications where contaminant-free conditions are critical.
3. Is this product compatible with nickel-NTA resin?
Absolutely, this Imidazole is specifically designed for use with nickel-NTA and cobalt resins in affinity purification of histidine-tagged proteins.
4. How should Imidazole 99.9% be stored for long-term use?
Store in a cool, dry place, tightly sealed in its original container to prevent moisture absorption and degradation. Avoid exposure to direct sunlight.
5. Are there any alternatives to Imidazole for protein elution?
Alternatives include histidine or lower concentrations of Imidazole, but these may require optimization as they can differ in elution efficiency and specificity.