Description
Specifications Table
| Product Name | Kanamycin Esculin Azide Broth |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of enterococci in microbiology |
Product Description
Kanamycin Esculin Azide Broth is a specialized microbiological medium designed for the selective isolation and identification of enterococci. This broth contains kanamycin, esculin, and sodium azide, which work together to inhibit gram-negative bacteria while allowing enterococci to grow and metabolize esculin. The hydrolysis of esculin produces a characteristic blackening of the medium, making it easy to identify positive results.
This broth is widely used in clinical and research laboratories for diagnostic purposes, particularly in the detection of enterococcal infections. It is also useful in environmental monitoring and food safety testing where enterococci may be present as indicators of fecal contamination.
When preparing Kanamycin Esculin Azide Broth, it is important to follow the manufacturer’s instructions carefully to ensure accurate results. The medium should be sterilized by autoclaving and then cooled before use. Once prepared, the broth can be inoculated with the sample and incubated at the appropriate temperature for the desired period.
For researchers and students working in microbiology, this broth is an essential tool for studying enterococci and their role in various infections. Its selective nature ensures that only the target bacteria grow, making it easier to identify and study them in detail.
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FAQs
What is Kanamycin Esculin Azide Broth used for?
Kanamycin Esculin Azide Broth is used for the selective isolation and identification of enterococci in clinical, environmental, and food samples.
How does Kanamycin Esculin Azide Broth work?
The broth contains kanamycin and sodium azide to inhibit gram-negative bacteria, while esculin allows enterococci to grow and produce a characteristic blackening of the medium.
What is the incubation period for Kanamycin Esculin Azide Broth?
The broth should be incubated at 35-37°C for 24-48 hours to observe the characteristic blackening indicating esculin hydrolysis.
Can Kanamycin Esculin Azide Broth be used for food safety testing?
Yes, this broth is commonly used in food safety testing to detect the presence of enterococci, which may indicate fecal contamination.
Where can I buy Kanamycin Esculin Azide Broth online?
You can buy Kanamycin Esculin Azide Broth online from eqipped, a trusted supplier of high-quality laboratory products.
