Description
Specifications Table
| Product Name | Kligler Iron Agar | 33943 | SRL Chem |
|---|---|
| Pack Size | 100 Gms | 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Microbial identification, sugar fermentation, H2S detection |
Product Description
Kligler Iron Agar is a versatile differential medium widely used in microbiology laboratories for identifying bacterial species based on their ability to ferment sugars and produce hydrogen sulfide. This medium contains lactose, glucose, and a pH indicator (phenol red) to detect acid production, along with ferrous sulfate to identify H2S-producing organisms.
Whether you’re a student conducting experiments or a researcher validating microbial cultures, Kligler Iron Agar provides clear, reliable results. Its dual-sugar system helps differentiate between lactose fermenters and non-fermenters, making it essential for diagnostic and educational purposes.
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How Kligler Iron Agar Works
The medium functions through two key reactions:
- Sugar Fermentation The presence of glucose and lactose allows bacteria to produce acid, turning the medium yellow due to the phenol red indicator.
- H2S Production Ferrous sulfate reacts with hydrogen sulfide, forming a black precipitate, which helps identify organisms like Salmonella and Proteus.
Applications of Kligler Iron Agar
This medium is commonly used for:
- Identifying enteric bacteria in clinical and environmental samples.
- Teaching microbiology students about metabolic pathways.
- Confirming the presence of H2S-producing pathogens in research.
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Storage and Handling
Store Kligler Iron Agar in a cool, dry place away from direct sunlight. Once prepared, the medium should be used within a specified period to ensure accuracy in results. Always follow standard lab protocols for handling microbial media.
Risk Note
This product is for laboratory use only. Handle with care, as improper use may lead to contamination or inaccurate results. Follow institutional safety guidelines when working with microbial cultures.
FAQs
What is Kligler Iron Agar used for?
Kligler Iron Agar is used to differentiate bacteria based on glucose/lactose fermentation and hydrogen sulfide production, aiding in microbial identification.
How do I prepare Kligler Iron Agar?
Dissolve the powder in distilled water, sterilize by autoclaving, and pour into sterile tubes or plates as per standard microbiological procedures.
Where can I buy Kligler Iron Agar online?
You can purchase lab-grade Kligler Iron Agar from eqipped, a trusted supplier of microbiology media in India.
What is the shelf life of Kligler Iron Agar?
The powder form has a long shelf life when stored properly, but prepared media should be used within the recommended timeframe for accurate results.
Is Kligler Iron Agar suitable for student labs?
Yes, it is commonly used in educational settings for teaching bacterial metabolism and identification techniques.
