Description
Specifications Table
Product Name – L-a-Glycerophosphate Oxidase (GPO) ex. microorganism 15U/mg
Quantity/Pack Size – 1000 units
Form – Lyophilized Powder
Grade – 15U/mg (Enzyme Activity)
Application – Biochemical Assays, Clinical Diagnostics, Metabolic Research
Product Overview
L-a-Glycerophosphate Oxidase (GPO) sourced from microorganisms is a highly active enzyme, precisely formulated to meet the rigorous demands of modern biochemical research and analytical applications. With a guaranteed specific activity of 15 units per milligram, this enzyme efficiently catalyzes the oxidation of L-a-glycerophosphate, yielding dihydroxyacetone phosphate and hydrogen peroxide. This critical reaction makes GPO an indispensable tool in numerous enzymatic assays, particularly for the quantitative determination of L-a-glycerophosphate in diverse biological samples. Its microbial origin ensures high purity and consistent performance, minimizing lot-to-lot variability and providing reliable results for critical experiments. Researchers, students, and educators can depend on the stability and robust functionality of this GPO for precise measurements in clinical diagnostics, metabolic pathway studies, and general biochemistry investigations. Our L-a-Glycerophosphate Oxidase is meticulously prepared to ensure maximum enzymatic activity and stability, offering a dependable reagent that facilitates accurate and reproducible outcomes in demanding laboratory environments. It represents a cornerstone component for establishing robust assay systems and advancing scientific understanding.
FAQs
1. What is the primary function of L-a-Glycerophosphate Oxidase?
L-a-Glycerophosphate Oxidase catalyzes the oxidation of L-a-glycerophosphate to dihydroxyacetone phosphate, simultaneously producing hydrogen peroxide. This reaction is crucial for various biochemical assays.
2. What are the typical applications of this GPO enzyme?
This GPO enzyme is widely used in biochemical assays for the determination of L-a-glycerophosphate, in clinical diagnostics, and in research involving metabolic pathways and lipid metabolism.
3. What is the recommended storage condition for this L-a-Glycerophosphate Oxidase?
For optimal stability and activity, L-a-Glycerophosphate Oxidase should be stored at -20°C. Ensure the vial is tightly sealed to prevent moisture absorption.
4. Can this GPO be used in coupled enzyme reactions?
Yes, the hydrogen peroxide produced by GPO makes it highly suitable for coupled enzyme reactions, often used with peroxidases and chromogenic substrates for colorimetric or fluorometric detection.
5. How is the activity of this GPO enzyme confirmed?
The activity of this L-a-Glycerophosphate Oxidase is rigorously confirmed through standard enzymatic assays that measure the rate of L-a-glycerophosphate oxidation under controlled conditions, ensuring a specific activity of 15U/mg.
