Description
Specifications Table
Product Name – L-Glutamate Dehydrogenase (GLDH) ex. Bovine Liver 290U/mg
Quantity/Pack Size – 10000unit
Form – Lyophilized Powder
Grade – Research Grade
Application – Enzymatic Assays, Metabolic Studies, Diagnostics
product overview
L-Glutamate Dehydrogenase (GLDH) from Bovine Liver, offered at a high activity of 290U/mg, is a pivotal enzyme for a wide range of biochemical investigations. This enzyme plays a crucial role in nitrogen metabolism, catalyzing the reversible oxidative deamination of L-glutamate to α-ketoglutarate and ammonia, utilizing NAD+ or NADP+ as a coenzyme. Its involvement in the interconversion of amino acids and keto acids makes it fundamental to understanding cellular energy metabolism and metabolic pathways. Sourced from bovine liver, this enzyme ensures consistent performance and high purity, essential for accurate and reproducible results in demanding laboratory environments. It is meticulously prepared to maintain optimal stability and enzymatic activity, providing researchers with a dependable tool for their experiments. Ideal for use in quantitative enzymatic assays, detailed metabolic studies, and the development of diagnostic applications, this GLDH offers exceptional quality for probing complex biological processes and ensuring robust analytical outcomes.
FAQs
1. What are the primary applications of this GLDH enzyme?
This L-Glutamate Dehydrogenase is primarily used in enzymatic assays, detailed metabolic pathway studies, and as a component in diagnostic kits, particularly for assessing liver function due to its role in ammonia detoxification.
2. What is the recommended storage condition for L-Glutamate Dehydrogenase?
For optimal stability and retention of enzymatic activity, L-Glutamate Dehydrogenase should be stored as recommended on the product label, typically at -20°C in its lyophilized form, protected from moisture.
3. Is this GLDH compatible with standard enzymatic assay buffers?
Yes, this L-Glutamate Dehydrogenase is designed for compatibility with common buffer systems used in enzymatic assays, often requiring buffers containing stabilizers and cofactors suitable for its activity.
4. How is the activity of L-Glutamate Dehydrogenase measured and expressed?
The activity of L-Glutamate Dehydrogenase is measured spectrophotometrically by monitoring the change in absorbance due to the reduction of NAD+ or NADP+ at 340 nm. It is typically expressed in Units per milligram (U/mg), where one Unit reduces one micromole of coenzyme per minute.
5. Can this enzyme be used to study ammonia metabolism?
Absolutely, L-Glutamate Dehydrogenase is a key enzyme in ammonia metabolism, directly involved in the detoxification of ammonia by converting it into glutamate, making it an excellent tool for studying these crucial biochemical processes.
