Description
Specifications Table
| Product Name | Luria Broth Base, Miller’s Modification, Granulated |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Granulated |
| Grade | Laboratory Grade |
| Application | Microbial culture, bacterial growth, research |
Product Description
Luria Broth Base, Miller’s Modification, is a granulated medium designed for the cultivation of bacteria, particularly E. coli. This formulation ensures optimal growth conditions, making it a staple in microbiology labs, educational institutions, and research facilities. The granulated form allows for easy preparation, saving time and reducing errors in media formulation.
Whether you’re a student setting up experiments or a researcher working on microbial studies, this Luria Broth Base provides consistent results. Its balanced composition supports robust bacterial growth, making it ideal for cloning, plasmid preparation, and general microbiology applications.
At eqipped, we ensure that every batch of Luria Broth Base meets stringent quality standards, so you can trust its performance in your lab. The 500 gm pack size is perfect for regular use, offering both convenience and cost-effectiveness.
Need reliable Luria Broth Base for your lab? Buy online today and experience seamless microbial culture results.
Why Luria Broth Base, Miller’s Modification?
This specific modification of Luria Broth is widely preferred in labs due to its enhanced nutrient profile. The addition of sodium chloride and adjusted peptone levels ensures better osmotic balance, promoting healthier bacterial colonies. Researchers and educators rely on this medium for its reproducibility and efficiency in supporting bacterial growth.
For students, this medium simplifies lab work, as its granulated form dissolves quickly and uniformly. No more clumping or inconsistent results—just reliable performance every time.
Applications
Luria Broth Base, Miller’s Modification, is used in:
- Bacterial culture and maintenance
- Plasmid DNA isolation
- Protein expression studies
- Antibiotic resistance testing
- Educational lab experiments
Its versatility makes it a must-have in any microbiology lab. Whether you’re culturing E. coli for cloning or studying bacterial metabolism, this medium delivers consistent results.
How to Use
Preparing Luria Broth is straightforward:
- Dissolve 25 grams of the granulated base in 1 liter of distilled water.
- Autoclave at 121°C for 15 minutes to sterilize.
- Cool to room temperature before inoculation.
For agar plates, simply add 15 grams of agar per liter before autoclaving. The medium is ready for use once solidified.
Storage and Handling
Store the granulated Luria Broth Base in a cool, dry place, away from direct sunlight. Once prepared, the liquid medium can be stored at 4°C for short-term use. For long-term storage, aliquot and freeze at -20°C.
Always follow standard lab safety protocols when handling microbial cultures. Wear appropriate PPE and dispose of waste according to lab guidelines.
FAQs
What is the shelf life of Luria Broth Base, Miller’s Modification?
The granulated form has a shelf life of 24 months when stored properly. Once prepared, use the liquid medium within a week for best results.
Can this medium be used for antibiotic selection?
Yes, Luria Broth Base is compatible with common antibiotics like ampicillin, kanamycin, and tetracycline for selection purposes.
Is this product suitable for student labs?
Absolutely. Its easy preparation and reliable performance make it ideal for educational settings.
Where can I buy Luria Broth Base online in India?
You can purchase it from eqipped, a trusted supplier of lab essentials with fast shipping across India.
What is the difference between Miller’s and Lennox modifications?
Miller’s modification contains higher sodium chloride (10 g/L) compared to Lennox (5 g/L), making it better for certain bacterial strains.
