Description
Specifications Table
| Product Name | Lysine Iron Cystine Broth Base |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Differential medium for lysine decarboxylase detection |
Product Description
Lysine Iron Cystine Broth Base is a specialized microbiological medium designed to detect lysine decarboxylase activity in bacteria. This medium is widely used in clinical and research laboratories to identify enteric pathogens, particularly those belonging to the Enterobacteriaceae family. The presence of lysine, iron, and cystine in the broth facilitates the differentiation of bacteria based on their ability to decarboxylate lysine, producing alkaline byproducts that can be visually observed.
When bacteria metabolize lysine, they produce cadaverine, which raises the pH of the medium. This pH change is indicated by the color shift of the bromocresol purple indicator from yellow to purple. The addition of iron and cystine further enhances the medium’s ability to support bacterial growth while providing a clear visual distinction between positive and negative reactions.
This broth base is particularly useful in identifying organisms like Salmonella and Shigella, which are lysine decarboxylase-negative, versus other enteric bacteria that may be positive. Its reliability and ease of use make it a staple in microbiology labs across India.
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How to Use Lysine Iron Cystine Broth Base
To prepare the medium, dissolve 35 grams of the powder in 1 liter of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. After cooling, dispense into sterile tubes and inoculate with the test organism. Incubate at 35-37°C for 18-24 hours. Observe for a color change from yellow to purple, indicating a positive lysine decarboxylase reaction.
Applications in Microbiology
Lysine Iron Cystine Broth Base is commonly used in:
- Clinical microbiology labs for bacterial identification
- Research settings to study bacterial metabolism
- Quality control in food and pharmaceutical industries
Its differential properties help in distinguishing between closely related bacterial species, making it an essential tool for accurate diagnostics.
Why This Medium Stands Out
Unlike generic broths, Lysine Iron Cystine Broth Base is formulated to provide clear and consistent results. The inclusion of cystine ensures optimal bacterial growth, while the iron component enhances the visibility of the reaction. This medium is trusted by professionals for its precision and reliability in microbiological testing.
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Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the broth should be used within a short period to maintain its efficacy. Always follow standard laboratory safety protocols when handling microbiological media.
FAQs
What is Lysine Iron Cystine Broth Base used for?
It is used to detect lysine decarboxylase activity in bacteria, helping identify enteric pathogens like Salmonella and Shigella.
How do I interpret the results?
A color change from yellow to purple indicates a positive lysine decarboxylase reaction.
Can this medium be used for other tests?
It is specifically designed for lysine decarboxylase detection and may not be suitable for other biochemical tests.
What is the shelf life of the powder?
The powder has a long shelf life when stored properly, but the prepared broth should be used promptly.
Where can I buy lysine iron cystine broth in India?
You can buy lysine iron cystine broth online from eqipped, a trusted supplier of Himedia microbiology products.
