Description
Specifications Table
| Product Name | M-Endo Broth MF (MF Endo Medium) |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Coliform detection in water and food samples |
Product Description
M-Endo Broth MF, also known as MF Endo Medium or M-Coliform Broth, is a specialized microbiological medium designed for the detection and enumeration of coliform bacteria. This medium is widely used in water testing, food safety, and environmental monitoring due to its ability to selectively isolate coliforms while inhibiting the growth of other microorganisms.
The medium contains lactose as the primary carbohydrate source, which coliform bacteria ferment to produce acid and gas. The presence of bile salts and brilliant green dye further enhances selectivity by suppressing non-coliform bacteria. The resulting colonies exhibit a characteristic metallic sheen, making identification straightforward.
Whether you’re a student, researcher, or educator, M-Endo Broth MF from eqipped ensures reliable results for your microbiology experiments. Its consistent performance and ease of use make it a staple in labs across India.
Key Applications
M-Endo Broth MF is commonly used in the following applications:
- Water quality testing for coliform contamination
- Food safety analysis to detect fecal coliforms
- Environmental monitoring of water sources
- Educational labs for microbiology training
Why This Medium?
Choosing the right medium is crucial for accurate coliform detection. M-Endo Broth MF stands out because of its:
- High selectivity for coliform bacteria
- Clear and distinguishable colony morphology
- Compatibility with standard microbiological techniques
- Consistent performance across different sample types
For labs looking to buy M-Endo Broth MF online, eqipped offers a trusted source with fast shipping and competitive pricing.
How to Use M-Endo Broth MF
Preparing and using M-Endo Broth MF is straightforward:
- Dissolve the powder in distilled water as per the manufacturer’s instructions.
- Sterilize the medium by autoclaving at 121°C for 15 minutes.
- Cool the medium to 45-50°C before pouring into sterile Petri dishes.
- Inoculate the medium with your sample and incubate at 35-37°C for 24-48 hours.
- Observe for characteristic coliform colonies with a metallic sheen.
Always follow standard lab safety protocols when handling microbiological media.
Storage and Handling
To maintain the integrity of M-Endo Broth MF, store the powder in a cool, dry place away from direct sunlight. Once prepared, the medium should be used immediately or stored at 2-8°C for short-term use. Avoid repeated freezing and thawing to prevent degradation.
Safety Considerations
While M-Endo Broth MF is generally safe for lab use, it is important to handle all microbiological media with care. Wear appropriate personal protective equipment (PPE), including gloves and lab coats, when preparing and using the medium. Dispose of used media and contaminated materials according to your institution’s biohazard waste guidelines.
FAQs
What is M-Endo Broth MF used for?
M-Endo Broth MF is used for the detection and enumeration of coliform bacteria in water, food, and environmental samples.
How do I prepare M-Endo Broth MF?
Dissolve the powder in distilled water, sterilize by autoclaving, and cool before use. Follow the manufacturer’s instructions for exact measurements.
What do coliform colonies look like on M-Endo Broth MF?
Coliform colonies typically exhibit a metallic sheen, making them easy to identify against the medium’s background.
Can I buy M-Endo Broth MF online in India?
Yes, you can buy M-Endo Broth MF online from eqipped, a trusted supplier of lab materials in India.
Is M-Endo Broth MF suitable for educational labs?
Absolutely. Its ease of use and clear results make it ideal for teaching microbiology concepts in educational settings.
