Description
Specifications Table
| Product Name | MacConkey Agar W/ Bromothymol Blue | 98384 | SRL Chem |
|---|---|
| Quantity/Pack Size | 100 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation and differentiation of lactose-fermenting bacteria |
Product Description
MacConkey Agar with Bromothymol Blue is a specialized microbiological medium designed for the selective isolation and differentiation of lactose-fermenting bacteria. This agar is widely used in clinical and research laboratories to identify Gram-negative enteric bacteria, particularly those from the Enterobacteriaceae family.
The addition of Bromothymol Blue enhances the differential capability of the medium. Lactose-fermenting colonies produce acid, turning the medium yellow, while non-lactose fermenters appear as colorless or pale colonies. This makes it easier to distinguish between bacterial species, aiding in accurate diagnosis and research.
Whether you’re a student, researcher, or educator, this high-quality agar from SRL Chem ensures reliable results in bacterial culture experiments. Its consistent performance and ease of use make it a staple in microbiology labs across India.
Need a reliable medium for bacterial culture? Buy MacConkey Agar with Bromothymol Blue online today for precise results.
How MacConkey Agar with Bromothymol Blue Works
This agar contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria, allowing only Gram-negative bacteria to thrive. The lactose in the medium serves as a fermentable carbohydrate, while Bromothymol Blue acts as a pH indicator.
When lactose is fermented, acid is produced, lowering the pH and causing the Bromothymol Blue to turn yellow. Non-lactose fermenters do not produce acid, so their colonies remain colorless or take on the natural color of the medium.
This differential staining is crucial for identifying pathogens like E. coli and Salmonella, making it an essential tool in microbiology.
Applications of MacConkey Agar with Bromothymol Blue
This medium is commonly used in:
- Clinical microbiology for identifying enteric pathogens
- Food and water testing for bacterial contamination
- Educational labs for teaching bacterial differentiation
- Research settings for studying Gram-negative bacteria
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Why This Agar is Essential for Your Lab
MacConkey Agar with Bromothymol Blue is a versatile medium that provides clear, distinguishable results. Its selective and differential properties make it indispensable for:
- Diagnosing infections caused by enteric bacteria
- Monitoring food and water safety
- Conducting microbiology experiments in academic settings
With its long shelf life and consistent performance, this agar is a cost-effective choice for labs of all sizes.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2–8°C and used within the recommended timeframe to ensure optimal performance.
Order MacConkey Agar with Bromothymol Blue online now and get fast delivery across India.
FAQs
What is the purpose of Bromothymol Blue in MacConkey Agar?
Bromothymol Blue acts as a pH indicator, turning yellow in the presence of acid produced by lactose-fermenting bacteria.
Can this agar be used for Gram-positive bacteria?
No, MacConkey Agar is selective for Gram-negative bacteria due to the presence of bile salts and crystal violet.
How should I prepare MacConkey Agar with Bromothymol Blue?
Suspend the powder in distilled water, heat to boiling, and sterilize by autoclaving. Cool to 45–50°C before pouring into plates.
What is the shelf life of this product?
The powder has a long shelf life when stored properly. Prepared plates should be used within a few weeks for best results.
Where can I buy MacConkey Agar with Bromothymol Blue in India?
You can buy it online from eqipped, a trusted supplier of lab-grade microbiological media.
Risk Note
This product is for laboratory use only. Handle with care, following standard microbiological safety protocols. Avoid inhalation and direct contact with skin or eyes.
