Description
Specifications Table
| Product Name | MacConkey Agar w/o C.V., NaCl w/ 0.5% Sodium Taurocholate |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of bile-tolerant Gram-negative bacteria |
Product Description
MacConkey Agar without crystal violet (C.V.) and sodium chloride (NaCl), supplemented with 0.5% sodium taurocholate, is a specialized microbiological medium designed for the selective isolation of bile-tolerant Gram-negative bacteria. This formulation enhances the growth of enteric pathogens while inhibiting most Gram-positive organisms, making it a critical tool in clinical and research laboratories.
Unlike traditional MacConkey Agar, this variant excludes crystal violet and salt, reducing potential toxicity while maintaining selectivity. The addition of sodium taurocholate further improves the recovery of fastidious organisms, ensuring accurate and reliable results in microbiological testing.
Whether you’re a student, researcher, or educator, this medium is essential for experiments involving bacterial identification, antibiotic susceptibility testing, or environmental monitoring. Its consistent performance and ease of preparation make it a preferred choice for laboratories across India.
Looking for high-quality MacConkey Agar without salt? Buy online at eqipped for authentic, lab-tested supplies.
Key Applications
This medium is widely used in:
- Clinical microbiology for isolating enteric pathogens like E. coli and Salmonella.
- Environmental testing to detect fecal contamination in water or food samples.
- Research studies on bacterial metabolism and antibiotic resistance.
- Educational labs for teaching bacterial culture techniques.
Why This Formulation?
The absence of crystal violet and sodium chloride reduces interference with certain bacterial strains, while sodium taurocholate acts as a bile salt substitute, promoting the growth of bile-tolerant organisms. This makes it ideal for:
- Isolating lactose-fermenting and non-fermenting Gram-negative bacteria.
- Differentiating between coliforms and non-coliforms based on colony color.
- Supporting the growth of fastidious organisms that may be inhibited by traditional MacConkey Agar.
Preparation and Usage
To prepare the medium, suspend 50 grams of the powder in 1 liter of distilled water. Heat to boiling to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45–50°C before pouring into sterile Petri dishes. Store prepared plates at 2–8°C.
Need MacConkey Agar for microbiology? Shop now at eqipped for fast, reliable delivery.
Safety and Handling
This product is for laboratory use only. Handle with standard microbiological safety precautions. Wear gloves, lab coats, and eye protection when preparing or using the medium. Dispose of contaminated materials according to local biosafety regulations.
FAQs
1. What is the difference between this MacConkey Agar and the standard version?
This variant lacks crystal violet and sodium chloride but includes 0.5% sodium taurocholate, making it less inhibitory to certain bacteria while maintaining selectivity for bile-tolerant organisms.
2. Can I use this medium for antibiotic susceptibility testing?
Yes, it is suitable for susceptibility testing of Gram-negative bacteria, provided the organism can grow in the absence of crystal violet and salt.
3. How should I store the powdered medium?
Store the unopened powder in a cool, dry place away from direct sunlight. Once prepared, store plates at 2–8°C and use within the recommended shelf life.
4. Is this medium suitable for isolating Salmonella?
Yes, it supports the growth of Salmonella and other enteric pathogens, though additional confirmatory tests may be needed for identification.
5. Where can I buy MacConkey Agar without salt online?
You can purchase authentic MacConkey Agar w/o C.V., NaCl w/ 0.5% Sodium Taurocholate from eqipped, ensuring quality and timely delivery.
