Description
Specifications Table
| Product Name | MacConkey Agar w/o C.V. w/ 0.5% Bile Salts | 64300 | SRL Chem |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, microbiology research |
Product Description
MacConkey Agar is a widely used selective and differential medium in microbiology labs. This variant, without crystal violet but with 0.5% bile salts, is specifically designed to inhibit the growth of Gram-positive bacteria while allowing Gram-negative bacteria to thrive. The addition of bile salts enhances its selectivity, making it ideal for isolating enteric pathogens like E. coli and Salmonella.
Whether you’re a student, researcher, or educator, this medium is essential for experiments involving bacterial identification and antibiotic susceptibility testing. The powder form ensures easy preparation and long shelf life, making it a cost-effective choice for labs of all sizes.
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MacConkey Agar is particularly useful in clinical and environmental microbiology. The medium’s ability to differentiate between lactose-fermenting and non-lactose-fermenting bacteria makes it a staple in diagnostic labs. The absence of crystal violet reduces potential toxicity, while the bile salts maintain high selectivity.
For best results, follow standard preparation protocols. Dissolve the powder in distilled water, sterilize by autoclaving, and pour into Petri dishes. Store the prepared plates at 2-8°C to maintain efficacy.
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FAQs
What is the difference between MacConkey Agar with and without crystal violet?
MacConkey Agar without crystal violet is less inhibitory to certain Gram-negative bacteria, making it more versatile for general use. The bile salts still provide selectivity against Gram-positive organisms.
Can I use this medium for antibiotic susceptibility testing?
Yes, MacConkey Agar is often used in susceptibility testing, especially for enteric bacteria. However, always follow standard protocols for accurate results.
What is the shelf life of MacConkey Agar powder?
The powder has a shelf life of 2-3 years when stored in a cool, dry place. Prepared plates should be used within 4 weeks.
How do I prepare MacConkey Agar from powder?
Dissolve 50 grams of powder in 1 liter of distilled water, heat to boiling, and autoclave at 121°C for 15 minutes. Cool to 45-50°C before pouring into plates.
Where can I buy MacConkey Agar online in India?
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