Description
Specifications Table
| Product Name | MacConkey Agar with CV and without NaCl |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, lactose fermentation studies |
Product Description
MacConkey Agar with CV and without NaCl is a specialized microbiological medium designed for the selective isolation and differentiation of Gram-negative bacteria. This formulation, enriched with crystal violet (CV) and devoid of sodium chloride (NaCl), enhances the growth of coliforms and enteric pathogens while inhibiting Gram-positive organisms. It is widely used in clinical, research, and educational laboratories for identifying lactose-fermenting and non-lactose-fermenting bacteria.
The absence of NaCl in this variant makes it particularly useful for applications where sodium interference is a concern. The medium’s differential properties allow for clear visualization of bacterial colonies, with lactose fermenters producing pink-red colonies due to acid production, while non-fermenters appear colorless or transparent.
Whether you’re a student conducting microbiology experiments or a researcher working on bacterial identification, this MacConkey Agar variant provides reliable results. Order your 100 gm pack today and ensure precise bacterial isolation in your lab.
FAQs
What is the difference between MacConkey Agar with and without NaCl?
The absence of NaCl in this variant reduces osmotic stress on bacteria, making it suitable for applications where sodium interference is undesirable. It maintains selectivity for Gram-negative organisms while enhancing differential properties.
Can this medium be used for environmental sampling?
Yes, it is commonly used in environmental microbiology to isolate coliforms and other Gram-negative bacteria from water, soil, and food samples.
How do I prepare MacConkey Agar with CV and without NaCl?
Dissolve 50 grams of the powder in 1 liter of distilled water, heat to boiling, and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into plates.
What bacteria can be isolated using this medium?
It selectively isolates Gram-negative bacteria such as Escherichia coli, Klebsiella, and Enterobacter, while inhibiting Gram-positive organisms like Staphylococcus.
Is this product suitable for educational labs?
Absolutely. Its differential properties make it an excellent tool for teaching bacterial identification and metabolic studies in academic settings.
