Description
Specifications Table
| Product Name | MacConkey Agar with CV, NaCl and 0.15% Bile Salts | M081A-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, especially Enterobacteriaceae |
Product Description
MacConkey Agar with CV, NaCl and 0.15% Bile Salts is a widely used microbiological medium designed for the selective isolation and differentiation of Gram-negative bacteria, particularly members of the Enterobacteriaceae family. This medium contains bile salts and crystal violet (CV), which inhibit the growth of Gram-positive bacteria while allowing Gram-negative bacteria to thrive. The addition of NaCl helps maintain osmotic balance, making it ideal for clinical and research applications.
This medium is essential for identifying lactose-fermenting bacteria, which produce pink or red colonies, from non-lactose fermenters, which appear colorless or transparent. It is commonly used in medical laboratories, educational institutions, and research facilities for diagnostic and experimental purposes.
When you buy MacConkey Agar with CV, NaCl and 0.15% Bile Salts online from eqipped, you get a high-quality product that meets stringent laboratory standards. Whether you’re a student, researcher, or educator, this medium ensures reliable results for your microbiology experiments.
How to Use MacConkey Agar with CV, NaCl and 0.15% Bile Salts
1. Preparation: Suspend 50 grams of the medium in 1 liter of distilled water. Heat gently while stirring to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes.
2. Pouring Plates: Cool the medium to 45-50°C and pour into sterile Petri dishes. Allow the agar to solidify before use.
3. Inoculation: Streak or spread the bacterial sample onto the agar surface using a sterile loop or swab.
4. Incubation: Invert the plates and incubate at 35-37°C for 18-24 hours.
5. Interpretation: Observe colony morphology and color changes to identify bacterial species.
Applications of MacConkey Agar
MacConkey Agar is widely used in:
- Clinical microbiology for identifying pathogens in urine, stool, and other samples.
- Food and water testing to detect bacterial contamination.
- Educational laboratories for teaching microbiology techniques.
- Research settings for studying bacterial growth and metabolism.
If you’re looking to buy MacConkey Agar with CV, NaCl and 0.15% Bile Salts online, eqipped offers a reliable and cost-effective solution for your laboratory needs.
Storage and Handling
Store the powdered medium in a cool, dry place away from direct sunlight. Once prepared, store the agar plates at 2-8°C and use within the recommended shelf life. Always follow standard laboratory safety protocols when handling microbiological media.
Risk Note
This product is for laboratory use only. Handle with care, as it may contain components that can cause irritation. Wear appropriate personal protective equipment (PPE) and follow biosafety guidelines when working with bacterial cultures.
FAQs
What is MacConkey Agar used for?
MacConkey Agar is used for the selective isolation and differentiation of Gram-negative bacteria, particularly Enterobacteriaceae. It helps identify lactose-fermenting and non-lactose-fermenting bacteria.
How does MacConkey Agar with CV and Bile Salts work?
The bile salts and crystal violet inhibit Gram-positive bacteria, while the lactose and neutral red dye allow differentiation of lactose-fermenting bacteria (pink/red colonies) from non-fermenters (colorless colonies).
Can I buy MacConkey Agar online in India?
Yes, you can buy MacConkey Agar with CV, NaCl and 0.15% Bile Salts online from eqipped, a trusted supplier of laboratory media and reagents.
What is the shelf life of MacConkey Agar?
The powdered medium has a shelf life of 2-3 years when stored properly. Prepared agar plates should be used within a few weeks for optimal results.
Is MacConkey Agar suitable for educational purposes?
Absolutely! This medium is commonly used in educational laboratories to teach students about bacterial isolation, differential media, and microbiological techniques.
