Description
Specifications Table
| Product Name | MacConkey Agar without CV, NaCl with 0.5% Bile Salts | M082A-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, especially Enterobacteriaceae |
Product Description
MacConkey Agar without CV, NaCl with 0.5% Bile Salts is a specialized microbiological medium designed for the selective isolation and differentiation of Gram-negative bacteria. This formulation, free from Crystal Violet (CV) and Sodium Chloride (NaCl), includes 0.5% bile salts to inhibit the growth of Gram-positive bacteria while allowing Gram-negative organisms to thrive.
This medium is particularly useful in clinical and research laboratories for identifying and studying enteric pathogens such as E. coli, Salmonella, and Shigella. The absence of CV and NaCl ensures that the medium remains selective without additional inhibitory effects, making it ideal for sensitive applications.
MacConkey Agar is widely used in microbiology for routine diagnostic procedures, environmental monitoring, and educational purposes. Its ability to differentiate lactose-fermenting and non-lactose-fermenting bacteria makes it a staple in any microbiology lab.
For researchers and students looking to buy MacConkey Agar online, eqipped offers a reliable and high-quality option with fast shipping across India.
Key Applications
MacConkey Agar without CV, NaCl with 0.5% Bile Salts is commonly used for:
- Isolating and identifying Gram-negative bacteria from clinical and environmental samples.
- Differentiating lactose-fermenting and non-lactose-fermenting bacteria.
- Routine microbiological testing in hospitals, research labs, and educational institutions.
- Quality control in food and water testing.
Why This Product Stands Out
This variant of MacConkey Agar is formulated without Crystal Violet and Sodium Chloride, reducing potential inhibitory effects on certain bacterial strains. The inclusion of 0.5% bile salts ensures selectivity while maintaining optimal growth conditions for target organisms.
Himedia’s M082A-500 gm pack provides a cost-effective solution for labs requiring bulk quantities without compromising on quality. Whether you’re a student, researcher, or lab technician, this medium delivers consistent and reliable results.
Usage Instructions
To prepare MacConkey Agar without CV, NaCl with 0.5% Bile Salts:
- Dissolve 50 grams of the powder in 1 liter of distilled water.
- Heat gently while stirring to ensure complete dissolution.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C and pour into sterile Petri dishes.
- Allow the medium to solidify before use.
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Safety and Storage
Store the powder in a cool, dry place away from direct sunlight. Once prepared, store the agar plates at 2-8°C and use within the recommended shelf life. Follow standard laboratory safety protocols when handling microbiological media.
FAQs
What is MacConkey Agar used for?
MacConkey Agar is used to selectively isolate and differentiate Gram-negative bacteria, particularly Enterobacteriaceae, in clinical and environmental samples.
Does this MacConkey Agar contain Crystal Violet (CV)?
No, this variant is formulated without Crystal Violet (CV) and Sodium Chloride (NaCl), making it suitable for sensitive applications.
Can I buy MacConkey Agar online in India?
Yes, eqipped offers MacConkey Agar without CV, NaCl with 0.5% Bile Salts for online purchase with fast delivery across India.
What is the shelf life of this product?
The powder has a shelf life of 2-3 years when stored properly. Prepared agar plates should be used within a few weeks for best results.
Is this product suitable for educational labs?
Absolutely. This medium is widely used in educational settings for teaching microbiology techniques and bacterial identification.
