Description
Specifications Table
| Product Name | MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate | M082-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, particularly enteric pathogens |
Product Description
MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate is a specialized microbial culture medium designed for the selective isolation of Gram-negative bacteria. Unlike traditional MacConkey Agar, this variant excludes crystal violet (CV) and sodium chloride (NaCl) while incorporating sodium taurocholate, a bile salt that enhances the growth of bile-tolerant organisms. This makes it particularly useful for isolating enteric pathogens like Escherichia coli and Salmonella species in clinical and research settings.
The absence of CV reduces inhibition of certain Gram-positive bacteria, while the addition of sodium taurocholate mimics the bile-rich environment of the intestinal tract, promoting the growth of bile-resistant organisms. This medium is widely used in microbiology labs for differential diagnosis, water testing, and food safety analysis.
At eqipped, we ensure this product meets stringent quality standards, providing researchers and students with reliable results. Whether you’re conducting routine microbial testing or advanced research, this agar offers consistent performance.
Key Applications
This medium is ideal for:
- Isolating and differentiating lactose-fermenting and non-lactose-fermenting Gram-negative bacteria.
- Diagnosing urinary tract infections and gastrointestinal infections.
- Environmental monitoring, including water and food sample testing.
- Educational purposes in microbiology labs for student training.
Why This Medium Stands Out
Unlike standard MacConkey Agar, this formulation provides a more permissive environment for bile-sensitive organisms while maintaining selectivity. The inclusion of sodium taurocholate ensures better recovery of enteric bacteria, making it a preferred choice for diagnostic labs.
Need a reliable medium for bacterial isolation? Buy MacConkey Agar without CV, NaCl with 0.5% Sodium Taurocholate online today for accurate results.
Usage Instructions
To prepare the medium, suspend 50 grams of the powder in 1 liter of distilled water. Heat gently to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a month for optimal performance.
FAQs
What is the difference between this MacConkey Agar and the standard version?
This variant excludes crystal violet (CV) and sodium chloride (NaCl) but includes 0.5% sodium taurocholate, making it more suitable for bile-tolerant Gram-negative bacteria.
Can this medium be used for antibiotic susceptibility testing?
Yes, it is compatible with standard susceptibility testing protocols for Gram-negative bacteria.
Is this product suitable for student lab experiments?
Absolutely. Its consistent performance makes it ideal for educational microbiology labs.
How long can prepared plates be stored?
Prepared plates should be used within 30 days when stored at 2-8°C.
Does eqipped offer bulk discounts on this product?
Yes, contact our support team for bulk pricing and institutional orders.
