Description
Specifications Table
| Product Name | MacConkey Agar without CV, with 1.2% Agar | M008B-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, differential lactose fermentation |
Product Description
When it comes to microbial isolation, precision matters. MacConkey Agar without CV is a trusted medium designed to selectively isolate Gram-negative bacteria while differentiating lactose fermenters from non-fermenters. Whether you’re a student setting up your first lab experiment or a researcher validating critical findings, this Himedia agar ensures consistent results every time.
Unlike traditional MacConkey Agar, this variant excludes crystal violet (CV), making it gentler on certain bacterial strains while maintaining its selective properties. The 1.2% agar concentration provides the perfect gel strength for clear colony visualization, reducing the risk of misinterpretation.
For educators, this agar is a classroom staple. Its ability to demonstrate bacterial metabolism through color changes (pink/red for lactose fermenters, colorless for non-fermenters) makes it an excellent teaching tool. Researchers rely on it for preliminary screening in clinical and environmental samples, where Gram-negative pathogens like E. coli or Salmonella need to be identified quickly.
Need a reliable supplier for MacConkey Agar without CV? eqipped offers authentic Himedia products with fast shipping across India.
One of the key advantages of this medium is its versatility. It supports the growth of a wide range of Gram-negative bacteria, including Enterobacteriaceae, while inhibiting most Gram-positive organisms. This makes it indispensable in food safety testing, water analysis, and clinical diagnostics.
Students often ask: Why choose MacConkey Agar without CV over the standard version? The answer lies in its broader compatibility. Some bacterial strains, especially those with delicate cell walls, may be inhibited by crystal violet. By removing CV, this formulation ensures you don’t miss out on isolating less robust but equally important pathogens.
At eqipped, we understand the frustration of receiving contaminated or expired media. That’s why we source directly from Himedia, ensuring each 500gm pack meets stringent quality standards. Our packaging is designed to preserve the agar’s efficacy, so you get consistent performance batch after batch.
Looking to buy MacConkey Agar online? eqipped provides lab-grade supplies with hassle-free ordering and secure payments.
For those working in resource-limited settings, this agar is cost-effective without compromising on quality. A single 500gm pack can prepare approximately 12-15 liters of medium, depending on your protocol. This makes it a budget-friendly choice for institutions running multiple experiments simultaneously.
Safety note: While MacConkey Agar without CV is generally safe for lab use, always follow standard microbiological practices. Wear appropriate PPE, sterilize equipment, and dispose of used plates as per biohazard guidelines. Avoid inhalation of powder and keep away from open flames.
FAQs
What is the difference between MacConkey Agar with and without CV?
MacConkey Agar without CV omits crystal violet, which can inhibit some Gram-negative bacteria. This makes it more inclusive for isolating a wider range of strains while still selectively suppressing Gram-positive organisms.
Can I use this agar for identifying E. coli?
Yes, this medium is excellent for E. coli identification. Lactose-fermenting strains like E. coli produce pink/red colonies, while non-fermenters (e.g., Salmonella) appear colorless.
How do I prepare MacConkey Agar without CV?
Suspend 50gm of powder in 1 liter of distilled water. Heat to boiling while stirring to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and pour into sterile plates.
What is the shelf life of this product?
The powder has a shelf life of 3 years from the date of manufacture when stored in a cool, dry place. Prepared plates should be used within 4 weeks if stored at 2-8°C.
Where can I buy authentic Himedia MacConkey Agar online in India?
eqipped offers genuine Himedia MacConkey Agar without CV with pan-India delivery. Order now for lab-ready supplies with assured quality.
