Description
Specifications Table
| Product Name | MacConkey Broth with Neutral Red |
|---|---|
| Quantity/Pack Size | 100gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Differentiation of lactose-fermenting bacteria |
Product Description
MacConkey Broth with Neutral Red is a widely used microbiological medium designed to differentiate between lactose-fermenting and non-lactose-fermenting bacteria. This selective medium contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria, allowing Gram-negative bacteria to thrive. The addition of neutral red as a pH indicator helps identify lactose fermentation by turning the broth yellow in the presence of acid-producing bacteria.
This medium is essential for clinical and research laboratories conducting bacterial identification tests. Whether you’re a student learning microbiology techniques or a researcher working on bacterial studies, MacConkey Broth with Neutral Red provides reliable results for your experiments.
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How to Use MacConkey Broth with Neutral Red
To prepare the medium, dissolve 100gm of the powder in 1 liter of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Allow the medium to cool before inoculating with your bacterial sample. Incubate at 35-37°C for 18-24 hours and observe the color change to determine lactose fermentation.
Applications of MacConkey Broth with Neutral Red
This medium is commonly used in:
- Clinical microbiology labs for bacterial identification
- Research studies on Gram-negative bacteria
- Educational institutions for teaching microbiology techniques
- Food and water testing for bacterial contamination
Why This Medium Matters in Microbiology
MacConkey Broth with Neutral Red plays a crucial role in distinguishing between different types of bacteria. The ability to identify lactose-fermenting bacteria is particularly important in clinical settings where quick and accurate bacterial identification can impact treatment decisions. For students and researchers, this medium provides a clear visual indication of bacterial metabolic activities.
Looking for reliable microbiology supplies? Shop MacConkey Broth with Neutral Red at eqipped for your lab needs.
Storage and Handling
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the medium should be used within a reasonable time frame to maintain its effectiveness. Always follow standard laboratory safety protocols when handling microbiological media.
Risk Note
This product is for laboratory use only. Handle with care as it contains biological components. Follow proper disposal procedures for microbiological waste.
FAQs
What is MacConkey Broth with Neutral Red used for?
It’s used to differentiate lactose-fermenting bacteria from non-lactose-fermenting bacteria in microbiology labs.
How do I prepare MacConkey Broth with Neutral Red?
Dissolve 100gm in 1L distilled water, autoclave at 121°C for 15 minutes, then cool before use.
What does a color change indicate in this medium?
A yellow color indicates lactose fermentation, while a red color suggests no fermentation.
Can I buy MacConkey Broth with Neutral Red in smaller quantities?
This product is available in 100gm packs. For bulk orders, contact eqipped’s customer support.
Is this medium suitable for educational purposes?
Yes, it’s commonly used in educational institutions for teaching bacterial identification techniques.
