Description
Specifications Table
| Product Name | MacConkey HiVeg Agar without CV, with 0.003% NR and 1.5% Agar |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective isolation of Gram-negative bacteria, lactose fermentation studies |
Product Description
MacConkey HiVeg Agar is a specialized microbiological medium designed for the selective isolation and differentiation of Gram-negative bacteria, particularly those capable of fermenting lactose. This variant, formulated without crystal violet (CV) and enriched with 0.003% neutral red (NR) and 1.5% agar, provides a reliable platform for microbiology research and diagnostic applications.
Unlike traditional MacConkey agar, this HiVeg version replaces animal-derived components with plant-based alternatives, making it suitable for vegan and cruelty-free laboratory practices. The absence of crystal violet ensures a clearer differentiation of lactose fermenters and non-fermenters, while the neutral red indicator enhances visibility of bacterial colonies.
Ideal for educational institutions, research labs, and clinical settings, this medium supports accurate identification of pathogens like E. coli and Klebsiella species. Its consistent performance and high purity make it a preferred choice for microbiologists across India.
Looking for a reliable MacConkey agar supplier? eqipped offers premium-quality media at competitive prices.
Key Applications
This medium is widely used in:
- Clinical microbiology for pathogen identification
- Environmental monitoring of water and food samples
- Educational demonstrations of bacterial metabolism
- Research studies on antibiotic resistance
Why This Medium Stands Out
The unique formulation of this MacConkey HiVeg Agar offers several advantages:
First, the exclusion of crystal violet reduces toxicity while maintaining selectivity. Second, the plant-based composition aligns with modern ethical laboratory standards. Finally, the optimized agar concentration ensures consistent gel strength for reliable results.
Need MacConkey agar 500gm for your lab? Order now for quick delivery and secure packaging.
Usage Guidelines
For optimal results, follow these preparation steps:
- Dissolve 50 grams of powder in 1 liter of distilled water
- Heat to boiling while stirring to ensure complete dissolution
- Autoclave at 121°C for 15 minutes
- Cool to 45-50°C before pouring into sterile Petri dishes
- Store prepared plates at 2-8°C in darkness
Safety Considerations
While this medium is generally safe for laboratory use, standard microbiological precautions should be observed:
- Wear appropriate PPE (gloves, lab coat, eye protection)
- Avoid inhalation of powder during preparation
- Dispose of used media according to biohazard waste protocols
FAQs
What is the shelf life of MacConkey HiVeg Agar?
The powder has a shelf life of 3 years when stored properly in a cool, dry place away from direct sunlight.
Can this medium be used for antibiotic susceptibility testing?
While primarily designed for isolation, it can support susceptibility testing when supplemented with appropriate antibiotics.
How does the absence of crystal violet affect performance?
Removing CV makes the medium less inhibitory to certain bacteria while maintaining its selective properties through bile salts.
Is this product suitable for student laboratory exercises?
Yes, its clear differentiation of lactose fermenters makes it excellent for educational demonstrations of bacterial metabolism.
What’s the typical incubation period for this medium?
Most results are visible within 18-24 hours of incubation at 35-37°C, though some slow-growing organisms may require 48 hours.
