Description
Specifications Table
| Product Name | MacConkey HiVeg Broth (Double Strength) with Neutral Red |
|---|---|
| Pack Size | 100 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Selective medium for lactose fermentation and Gram-negative bacteria isolation |
Product Description
MacConkey HiVeg Broth (Double Strength) with Neutral Red is a specialized microbiological medium designed for the selective isolation and differentiation of lactose-fermenting Gram-negative bacteria. This double-strength formulation enhances sensitivity, making it ideal for research, clinical diagnostics, and educational labs.
The inclusion of Neutral Red as a pH indicator allows for clear visual differentiation of lactose fermenters (pink/red colonies) from non-fermenters (colorless). As a HiVeg variant, it replaces animal-derived components with plant-based alternatives, ensuring ethical and consistent results.
Whether you’re a student, researcher, or educator, this medium is a reliable choice for experiments involving enteric bacteria like E. coli and Klebsiella. Its powdered form ensures long shelf life and easy preparation.
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Key Applications
- Differentiation of lactose-fermenting and non-fermenting bacteria
- Isolation of Gram-negative enteric pathogens
- Quality control in food and water microbiology
- Educational demonstrations in bacteriology
Why This Medium?
Unlike traditional MacConkey broth, this double-strength variant provides higher selectivity, reducing false negatives. The HiVeg formulation aligns with modern lab practices, avoiding animal-derived peptides while maintaining performance. Neutral Red’s sharp color change simplifies interpretation, saving time in busy labs.
For researchers, the consistency of Himedia’s manufacturing ensures reproducible results. Students benefit from its straightforward protocol, making it a staple in microbiology curricula.
Preparation and Usage
Reconstitute the powder in distilled water as per the manufacturer’s instructions. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 45–50°C before adding supplements (if required). Pour into sterile plates or tubes and allow to solidify.
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Storage and Handling
Store the unopened powder at 2–8°C in a dry place. Prepared medium should be used within 7 days if stored refrigerated. Avoid freeze-thaw cycles to maintain integrity.
Safety Note
This product is for laboratory use only. Handle with standard microbiological safety precautions. Wear gloves and lab coats, and dispose of waste according to biohazard protocols.
FAQs
What is the difference between MacConkey broth and HiVeg broth?
MacConkey HiVeg Broth replaces animal-derived peptides with plant-based alternatives, making it suitable for vegan labs while maintaining identical performance to traditional formulations.
Can this medium be used for antibiotic susceptibility testing?
While primarily a selective medium, it can support susceptibility tests for lactose-fermenting bacteria. However, specialized media like Mueller-Hinton are recommended for standardized AST.
How long does the color change take to appear?
Lactose fermentation typically produces visible color changes (pink/red) within 18–24 hours of incubation at 37°C. Non-fermenters remain colorless.
Is this product suitable for student labs?
Yes, its clear differentiation and ease of preparation make it ideal for educational settings. eqipped offers bulk discounts for institutions.
Where can I buy authentic Himedia MacConkey HiVeg Broth in India?
eqipped is an authorized supplier of Himedia products, ensuring genuine, high-quality media with pan-India delivery.
