Description
Specifications Table
| Product Name | MacConkey Sorbitol Agar Base |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Detection of E. coli O157:H7 |
Product Description
MacConkey Sorbitol Agar Base is a specialized microbiological medium designed for the selective isolation and differentiation of E. coli O157:H7. This agar is widely used in clinical and food microbiology labs due to its ability to inhibit gram-positive bacteria while allowing sorbitol-fermenting and non-fermenting organisms to grow. The presence of bile salts and crystal violet makes it selective, while the sorbitol and neutral red indicator help in differentiating E. coli strains.
If you’re looking to buy MacConkey Sorbitol Agar Base online, eqipped offers a reliable supply of this high-quality medium. Whether you’re a student, researcher, or educator, this product ensures accurate and efficient detection of pathogenic E. coli. The 500gm pack size is ideal for labs with moderate to high testing volumes, providing cost-effective solutions without compromising on quality.
For those searching for MacConkey Sorbitol Agar price or a trusted MacConkey Sorbitol Agar base supplier, eqipped delivers competitive pricing and fast shipping across India. The medium is easy to prepare and provides clear, interpretable results, making it a staple in diagnostic and research settings.
Key Applications
This agar base is primarily used for:
- Detection of E. coli O157:H7 in food and clinical samples
- Differentiation of sorbitol-fermenting and non-fermenting bacteria
- Research in microbiology and infectious disease studies
When you buy MacConkey Sorbitol Agar online from eqipped, you get a product that meets stringent quality standards. The medium is tested for performance and consistency, ensuring reliable results every time. Whether you’re setting up a new lab or restocking, this agar base is a smart choice for accurate bacterial identification.
Why This Product Stands Out
MacConkey Sorbitol Agar Base from Himedia is formulated to provide optimal growth conditions for target organisms while suppressing unwanted flora. The inclusion of sorbitol instead of lactose makes it particularly effective for detecting non-sorbitol fermenting E. coli strains, which are often pathogenic. This makes it an essential tool for food safety testing and clinical diagnostics.
For educators and students, this medium offers a practical way to demonstrate bacterial metabolism and selective media principles. Its clear differentiation between colonies makes it an excellent teaching aid in microbiology courses.
Preparation and Usage
To prepare the medium, suspend 50 grams of the powder in 1 liter of distilled water. Heat to boiling to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes. Once set, the plates are ready for inoculation.
Incubate the inoculated plates at 35-37°C for 18-24 hours. Sorbitol-fermenting colonies appear pink to red, while non-fermenting colonies (like E. coli O157:H7) remain colorless. This clear visual distinction simplifies identification and reduces the need for additional confirmatory tests.
Safety and Storage
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable timeframe to ensure optimal performance. Always follow standard laboratory safety protocols when handling microbiological media.
FAQs
What is MacConkey Sorbitol Agar Base used for?
It is used for the selective isolation and differentiation of E. coli O157:H7, particularly in food and clinical samples.
How do I prepare MacConkey Sorbitol Agar?
Suspend 50 grams in 1 liter of distilled water, boil to dissolve, autoclave at 121°C for 15 minutes, cool to 45-50°C, and pour into sterile plates.
Where can I buy MacConkey Sorbitol Agar Base online?
You can buy it from eqipped, a trusted supplier of laboratory media and reagents in India.
What is the shelf life of this product?
The powder has a long shelf life when stored properly, typically 2-3 years from the date of manufacture.
Can this medium be used for teaching purposes?
Yes, it is an excellent tool for demonstrating bacterial metabolism and selective media principles in educational settings.
