Description
Specifications Table
| Product Name | MacConkey Sorbitol Agar Base with Rhamnose |
|---|---|
| Pack Size | 500 gm |
| Form | Powder |
| Grade | Laboratory/Microbiology Grade |
| Application | Differentiation and isolation of E. coli O157:H7 |
Product Description
MacConkey Sorbitol Agar Base with Rhamnose (M1727) is a specialized microbiological medium designed for the selective isolation and differentiation of Escherichia coli O157:H7. This formulation combines the principles of MacConkey agar with sorbitol fermentation and rhamnose utilization, making it an essential tool for clinical and research laboratories.
The medium inhibits the growth of Gram-positive bacteria while allowing Gram-negative bacteria to thrive. Sorbitol-fermenting colonies appear pink, while non-fermenters (like E. coli O157:H7) remain colorless, aiding in rapid identification. The addition of rhamnose enhances selectivity, ensuring accurate results in food safety testing and medical diagnostics.
At eqipped, we provide high-purity MacConkey Sorbitol Agar Base with Rhamnose, ensuring consistent performance for your critical applications. Whether you’re a student, researcher, or educator, this medium is ideal for experiments requiring precise bacterial differentiation.
Key Applications
This agar base is widely used in:
- Clinical microbiology for pathogen detection
- Food safety testing for E. coli contamination
- Research studies on bacterial metabolism
- Educational labs for microbiology training
Need reliable microbiology media? Buy MacConkey Sorbitol Agar Base with Rhamnose online at eqipped for guaranteed quality and fast delivery.
Preparation and Usage
To prepare the medium, suspend 50 grams of the powder in 1 liter of distilled water. Heat to boiling to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C before pouring into sterile Petri dishes.
For best results, store the prepared medium at 2-8°C and use within the recommended shelf life. Always follow standard microbiological practices when handling and disposing of cultures.
Safety Information
This product is for laboratory use only. Handle with care, wearing appropriate personal protective equipment (PPE). Avoid inhalation or direct contact with skin and eyes. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists.
FAQs
What is MacConkey Sorbitol Agar Base with Rhamnose used for?
It is used to selectively isolate and differentiate E. coli O157:H7 from other bacteria, particularly in food and clinical samples.
How does sorbitol fermentation help in identification?
Sorbitol-fermenting bacteria produce acid, turning the medium pink, while non-fermenters like E. coli O157:H7 remain colorless.
Can this medium be used for routine microbiology labs?
Yes, it is suitable for both research and educational settings where bacterial differentiation is required.
What is the shelf life of MacConkey Sorbitol Agar Base with Rhamnose?
The powder has a shelf life of 2-3 years when stored in a cool, dry place. Prepared plates should be used within 4 weeks.
Where can I buy MacConkey Sorbitol Agar Base with Rhamnose online?
You can purchase it from eqipped, a trusted supplier of laboratory media and reagents in India.
