Description
Specifications Table
| Product Name | MacConkey Sorbitol HiCynth Agar (Sorbitol HiCynth Agar) | MCD298-500 gm Himedia |
|---|---|
| Quantity/Pack Size | 500 gm |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation and differentiation of E. coli O157:H7 |
Product Description
MacConkey Sorbitol HiCynth Agar is a specialized microbiological medium designed for the selective isolation and differentiation of Escherichia coli O157:H7, a pathogenic strain responsible for severe foodborne illnesses. This agar incorporates sorbitol as the primary carbohydrate source, allowing for the differentiation of sorbitol-fermenting and non-fermenting strains. The addition of bile salts and crystal violet inhibits the growth of Gram-positive bacteria, ensuring selective isolation of Gram-negative organisms.
This medium is widely used in clinical, food, and environmental microbiology laboratories for the detection of E. coli O157:H7, which does not ferment sorbitol and appears as colorless colonies on the agar. Sorbitol-fermenting strains, on the other hand, produce pink or red colonies due to the pH indicator neutral red. The inclusion of HiCynth enhances the visibility of colonies, making interpretation easier and more accurate.
Whether you’re conducting research, quality control, or diagnostic testing, MacConkey Sorbitol HiCynth Agar from eqipped provides reliable and consistent results. Its high-quality formulation ensures optimal performance, making it a trusted choice for microbiologists across India.
Key Applications
MacConkey Sorbitol HiCynth Agar is essential for:
- Detection of E. coli O157:H7 in food samples
- Clinical diagnostics for pathogenic E. coli strains
- Environmental monitoring for microbial contamination
- Research studies on bacterial metabolism and virulence
For accurate and reliable E. coli detection, buy MacConkey Sorbitol HiCynth Agar online from eqipped today.
Why This Agar Stands Out
This medium is formulated with precision to ensure high selectivity and differentiation. The combination of sorbitol, bile salts, and crystal violet creates an environment where only the target organisms can thrive. The HiCynth component further improves colony visibility, reducing the risk of misinterpretation. Unlike traditional MacConkey agar, this variant is specifically optimized for the detection of non-sorbitol-fermenting E. coli strains, making it indispensable in modern microbiology labs.
When you buy MacConkey Sorbitol HiCynth Agar from eqipped, you’re choosing a product backed by rigorous quality control and trusted by professionals in the field.
Usage Guidelines
To achieve the best results with MacConkey Sorbitol HiCynth Agar, follow these steps:
- Suspend 50 grams of the powder in 1 liter of distilled water.
- Heat to boiling to dissolve the medium completely.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Cool to 45-50°C and pour into sterile Petri dishes.
- Inoculate the plates and incubate at 35-37°C for 18-24 hours.
Observe the colonies for color changes. Non-sorbitol-fermenting E. coli O157:H7 will appear as colorless colonies, while sorbitol-fermenting strains will be pink or red.
Safety and Storage
MacConkey Sorbitol HiCynth Agar is generally safe for laboratory use. However, always follow standard microbiological practices when handling bacterial cultures. Store the powder in a cool, dry place, away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable timeframe to maintain efficacy.
FAQs
What is the primary use of MacConkey Sorbitol HiCynth Agar?
This agar is primarily used for the selective isolation and differentiation of E. coli O157:H7, a pathogenic strain that does not ferment sorbitol.
How does MacConkey Sorbitol HiCynth Agar differ from standard MacConkey agar?
Unlike standard MacConkey agar, this variant uses sorbitol instead of lactose, allowing for the differentiation of sorbitol-fermenting and non-fermenting strains of E. coli.
What do the colony colors indicate on this agar?
Non-sorbitol-fermenting E. coli O157:H7 appears as colorless colonies, while sorbitol-fermenting strains produce pink or red colonies due to the pH indicator.
Can this agar be used for other bacteria besides E. coli?
While designed for E. coli O157:H7, it can also support the growth of other Gram-negative bacteria, though it is not optimized for their differentiation.
Where can I buy MacConkey Sorbitol HiCynth Agar online in India?
You can purchase high-quality MacConkey Sorbitol HiCynth Agar from eqipped, ensuring fast delivery and reliable performance for your lab needs.
