Description
Specifications Table
| Product Name | Mannitol Salt HiVeg Agar Base |
|---|---|
| Pack Size | 100g |
| Form | Powder |
| Grade | Microbiology Grade |
| Application | Selective isolation of staphylococci |
Product Description
Mannitol Salt HiVeg Agar Base is a specialized microbiological medium designed for the selective isolation and differentiation of pathogenic staphylococci, particularly Staphylococcus aureus. This agar base is formulated with mannitol as the fermentable carbohydrate and a high salt concentration (7.5%) to inhibit the growth of most bacteria except staphylococci. The HiVeg variant ensures it is free from animal-derived components, making it suitable for vegan and ethical research applications.
This medium is widely used in clinical microbiology, food safety testing, and research laboratories to identify and study staphylococcal infections. The presence of phenol red as a pH indicator allows for easy differentiation of mannitol-fermenting staphylococci (yellow colonies) from non-fermenters (red colonies).
At eqipped, we provide high-quality microbiology media to support accurate and reliable research outcomes. Whether you’re a student, researcher, or educator, this agar base is an essential tool for your lab experiments.
Key Applications
Mannitol Salt HiVeg Agar Base is commonly used for:
- Isolating Staphylococcus aureus from clinical samples
- Food microbiology testing for staphylococcal contamination
- Educational purposes in microbiology labs
- Research on bacterial pathogenesis and antibiotic resistance
Why This Agar Base?
This medium is preferred for its high selectivity and differential capabilities. The 7.5% sodium chloride concentration creates a hypertonic environment that inhibits most bacteria, allowing staphylococci to thrive. The mannitol fermentation test helps distinguish pathogenic strains, making it invaluable for diagnostic and research purposes.
For researchers and students working with bacterial cultures, having a reliable medium like this ensures accurate results. If you’re looking to buy Mannitol Salt HiVeg Agar Base online, eqipped offers a seamless purchasing experience with authentic Himedia products.
Usage Instructions
To prepare the medium, suspend 110 grams of the powder in 1 liter of distilled water. Heat to boiling to dissolve completely, then sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and pour into sterile Petri dishes. Allow the agar to solidify before use.
Inoculate the plates with your sample and incubate at 35-37°C for 24-48 hours. Observe for characteristic colony morphology and color changes to identify staphylococcal species.
Safety and Storage
Store the powder in a cool, dry place away from direct sunlight. Once prepared, the agar plates should be stored at 2-8°C and used within a reasonable timeframe to maintain efficacy.
FAQs
What is Mannitol Salt HiVeg Agar Base used for?
It is used for the selective isolation and differentiation of pathogenic staphylococci, particularly Staphylococcus aureus, in clinical and research settings.
How does Mannitol Salt Agar differentiate bacteria?
The medium contains mannitol and phenol red. Staphylococcus aureus ferments mannitol, producing acid that turns the medium yellow, while non-fermenters leave it red.
Can this agar base be used for food testing?
Yes, it is commonly used in food microbiology to detect staphylococcal contamination in food samples.
Is this product suitable for vegan research?
Yes, the HiVeg variant is free from animal-derived components, making it ideal for ethical research.
Where can I buy Mannitol Salt HiVeg Agar Base online?
You can purchase authentic Himedia Mannitol Salt HiVeg Agar Base online at eqipped with fast delivery across India.
