Description
Specifications Table
| Product Name | MES Buffer For Biochemistry | Q10362 | Qualigens |
|---|---|
| Quantity/Pack Size | 25gm |
| Form | Powder |
| Grade | Laboratory |
| Application | Biochemistry, protein research, electrophoresis |
Product Description
MES buffer, or 2-(N-morpholino)ethanesulfonic acid, is a widely used buffering agent in biochemistry and molecular biology. This high-purity MES buffer from Qualigens is designed for applications requiring precise pH control, such as protein purification, cell culture, and gel electrophoresis. Its non-toxic and non-reactive properties make it ideal for sensitive biological systems.
Whether you’re a student setting up experiments or a researcher optimizing protocols, this MES buffer ensures consistent performance. The 25gm pack size is perfect for small-scale lab work, reducing waste while providing enough material for multiple experiments. For larger requirements, eqipped offers bulk options to meet your needs.
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MES buffer is particularly useful in maintaining a stable pH range of 5.5 to 6.7, which is critical for enzymatic reactions and protein stability. Unlike other buffers, MES does not chelate metal ions, making it compatible with a wide range of biochemical assays. Its water solubility and low UV absorbance further enhance its utility in spectroscopic studies.
For researchers working with cell cultures, MES buffer helps maintain optimal conditions without interfering with cellular processes. Students can rely on its consistency for reproducible results in lab experiments. Whether you’re studying protein interactions or running Western blots, this buffer is a staple in any biochemistry lab.
Applications of MES Buffer
MES buffer is versatile and finds use in various laboratory applications:
In protein research, it stabilizes pH during purification and crystallization. For electrophoresis, it ensures sharp band resolution in gels. Cell biologists use it to maintain physiological pH in culture media. Its compatibility with common lab reagents makes it a go-to choice for many protocols.
When buying MES buffer online, quality matters. Qualigens is a trusted name in lab chemicals, and eqipped ensures you receive genuine products with proper documentation. Each batch is tested for purity and performance, so you can focus on your research without worrying about reagent quality.
Why MES Buffer is Essential in Biochemistry
Biochemical reactions are highly sensitive to pH changes. Even minor fluctuations can denature proteins or alter enzyme activity. MES buffer’s effective range (5.5–6.7) covers the pH of many cellular compartments, making it indispensable for in vitro studies mimicking physiological conditions.
Unlike phosphate buffers, MES does not precipitate with calcium or magnesium ions, which is crucial for experiments involving metal-dependent enzymes. Its zwitterionic nature also minimizes interactions with biological molecules, ensuring accurate results.
Looking for a trusted MES buffer supplier? Order Qualigens MES buffer online at eqipped today.
Handling and Storage
Store MES buffer in a cool, dry place away from direct sunlight. Keep the container tightly sealed to prevent moisture absorption. For long-term storage, desiccants can be used to maintain product integrity. Always wear appropriate personal protective equipment (PPE) when handling chemicals.
Safety Information
MES buffer is generally safe when used as directed. However, avoid inhalation or direct contact with skin and eyes. In case of accidental exposure, rinse thoroughly with water and seek medical advice if irritation persists. Refer to the Safety Data Sheet (SDS) for detailed handling instructions.
FAQs
What is MES buffer used for in biochemistry?
MES buffer is primarily used to maintain a stable pH in biochemical experiments, particularly in protein research, cell culture, and electrophoresis. Its effective range of 5.5–6.7 makes it ideal for applications requiring slightly acidic conditions.
How do I prepare a 100mM MES buffer solution?
To prepare a 100mM MES buffer, dissolve 1.95g of MES powder in 100ml of distilled water. Adjust the pH to the desired value using NaOH or HCl, then sterilize by filtration if needed for cell culture applications.
Is MES buffer compatible with cell culture?
Yes, MES buffer is commonly used in cell culture media to maintain physiological pH without toxicity. Its non-chelating properties make it suitable for metal-ion-dependent cellular processes.
Where can I buy MES buffer online in India?
You can buy authentic Qualigens MES buffer online at eqipped. We offer lab-grade chemicals with fast delivery across India, ensuring you get reliable reagents for your research.
What is the shelf life of MES buffer powder?
When stored properly in a cool, dry place, MES buffer powder has a shelf life of at least 2 years. Always check the manufacturer’s expiry date and store as directed to maintain product quality.
