Specifications Table
Product Name – METHYLENE BLUE HYDRATE 82% For Microscopy
Quantity/Pack Size – 25 g, 100 g, 1000 g
Form – Powder
Grade – For Microscopy
Application – Staining, microscopy, biological research
Product Overview
Methylene Blue Hydrate 82% is a high-purity, water-soluble dye specifically formulated for microscopy applications. Known for its deep blue coloration, this lab-grade reagent provides exceptional staining clarity, making it ideal for visualizing cellular structures, bacteria, and tissue samples under a microscope. The hydrated form ensures stability and consistent performance, reducing variability in experimental results. Its high concentration (82%) guarantees strong, reliable staining without the need for excessive quantities, ensuring cost-efficiency for frequent lab use. The powder form allows for easy dissolution in aqueous solutions, enabling precise preparation of staining solutions tailored to specific experimental requirements. Whether used in histological studies, bacteriological examinations, or general microscopy, this Methylene Blue Hydrate delivers sharp contrast and clear differentiation of cellular components, enhancing the accuracy of observations. The product is rigorously tested to meet microscopy-grade standards, ensuring minimal impurities and maximum reliability in research and educational settings. Its versatility extends to compatibility with various staining protocols, making it a staple in laboratories focused on biological and medical research.
FAQs
1. What is the solubility of Methylene Blue Hydrate 82% in water?
Methylene Blue Hydrate 82% is highly soluble in water, typically dissolving at a concentration of up to 30-40 mg/mL, depending on the temperature and pH of the solution. For best results, use distilled or deionized water to prepare the staining solution.
2. Can this Methylene Blue Hydrate be used for Gram staining?
While Methylene Blue Hydrate is not the primary stain used in the traditional Gram staining procedure, it can serve as a counterstain or alternative in modified protocols. For standard Gram staining, Crystal Violet is typically preferred as the primary stain.
3. How should Methylene Blue Hydrate 82% be stored to maintain its stability?
Store the product in a tightly sealed container, protected from light and moisture, at room temperature (15-25?C). Exposure to direct sunlight or humidity can degrade the dye, reducing its effectiveness over time.
4. Is this Methylene Blue Hydrate suitable for live cell staining?
Methylene Blue Hydrate can be used for live cell staining, but its application should be carefully controlled, as high concentrations or prolonged exposure may be cytotoxic. It is commonly used at lower concentrations for viability assays or vital staining.
5. Are there any safety precautions to consider when handling this product?
Yes, Methylene Blue Hydrate should be handled with care, using appropriate personal protective equipment (PPE) such as gloves, lab coats, and safety goggles. Avoid inhalation or direct contact with skin and eyes, as it may cause irritation. Work in a well-ventilated area or under a fume hood when preparing solutions.