Description
Specifications Table
| Product Name | Miller Luria Bertani Broth | M1245-100G | Himedia |
|---|---|
| Quantity/Pack Size | 100 gm |
| Form | Powder |
| Grade | Molecular Biology Grade |
| Application | Bacterial culture, cloning, protein expression |
Product Description
Miller Luria Bertani Broth, commonly known as LB broth, is a nutrient-rich medium widely used in microbiology laboratories for the cultivation of bacteria. This high-quality formulation from Himedia ensures optimal growth conditions for a variety of bacterial strains, making it indispensable for research, educational labs, and biotechnology applications.
Whether you’re a student working on a microbiology project or a researcher conducting experiments, this LB broth provides the essential nutrients bacteria need to thrive. The powdered form allows for easy preparation, and its molecular biology grade guarantees consistency and reliability in your results.
At eqipped, we understand the importance of dependable lab supplies. That’s why we offer this premium Miller Luria Bertani Broth at competitive prices, ensuring you get the best value without compromising on quality. Need LB broth for your next experiment? Order now and get it delivered to your lab quickly.
Key Applications of LB Broth
LB broth is versatile and supports various applications in microbiology and molecular biology. Here are some common uses:
Bacterial Culture: Provides an ideal environment for the growth of E. coli and other bacterial strains, essential for cloning and protein expression studies.
Plasmid DNA Isolation: Used in the initial steps of plasmid extraction protocols to amplify bacterial cultures.
Antibiotic Resistance Testing: Helps in determining the susceptibility of bacteria to different antibiotics.
Research and Education: A staple in academic labs for teaching microbiology techniques and conducting experiments.
If you’re looking to buy LB broth online, this product is an excellent choice for both beginners and experienced researchers.
Why LB Broth is Essential for Your Lab
Choosing the right culture medium is crucial for accurate and reproducible results. Miller Luria Bertani Broth stands out because of its balanced composition, which includes:
Tryptone: A source of amino acids and nitrogen, promoting robust bacterial growth.
Yeast Extract: Provides vitamins and other essential nutrients.
Sodium Chloride: Maintains osmotic balance, ensuring optimal conditions for bacterial cells.
This combination makes LB broth a go-to medium for routine bacterial culture and more complex applications. Whether you’re preparing competent cells or screening recombinant colonies, this broth delivers consistent performance.
For those searching for LB broth price or a reliable Miller LB broth supplier, eqipped offers a seamless purchasing experience with genuine Himedia products.
Preparation and Storage Guidelines
To prepare LB broth, dissolve 20 grams of the powder in 1 liter of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Once cooled, the medium is ready for use. Store the powder in a cool, dry place, away from direct sunlight, to maintain its efficacy.
Always follow standard lab protocols when handling and disposing of bacterial cultures to ensure safety.
Safety and Handling
While LB broth is generally safe to handle, it’s important to follow basic lab safety practices:
Wear appropriate personal protective equipment (PPE) such as gloves and lab coats.
Avoid inhaling the powder to prevent respiratory irritation.
Dispose of used media and contaminated materials according to your institution’s biohazard waste guidelines.
For high-risk applications, ensure proper sterilization and containment procedures are in place.
FAQs
What is the shelf life of Miller Luria Bertani Broth?
The powdered form has a shelf life of 24 months when stored under recommended conditions. Once prepared, the liquid medium should be used within a few days or stored at 4°C for short-term use.
Can I use LB broth for fungal cultures?
LB broth is specifically formulated for bacterial growth and may not support fungal cultures effectively. For fungi, consider using media like Potato Dextrose Agar (PDA).
How do I adjust the pH of LB broth?
The standard pH of LB broth is around 7.0. If adjustment is needed, use sterile 1N NaOH or 1N HCl before autoclaving. Always verify pH after sterilization.
Is this LB broth suitable for antibiotic selection?
Yes, this LB broth can be supplemented with antibiotics like ampicillin or kanamycin for selection purposes. Ensure proper aseptic techniques when adding antibiotics.
Where can I buy LB broth online in India?
You can purchase authentic Miller Luria Bertani Broth (M1245-100G) from eqipped. We offer competitive pricing and reliable delivery across India.
