Description
Specifications Table
| Product Name | Minimum Essential Medium Eagle (MEM) | AL047S-6x500ML | Himedia |
|---|---|
| Quantity/Pack Size | 6 × 500 ml |
| Form | Liquid |
| Grade | Cell Culture Grade |
| Application | Mammalian cell culture, research, and diagnostic applications |
Product Description
Minimum Essential Medium Eagle (MEM) is a widely used cell culture medium designed to support the growth of various mammalian cells. Developed by Harry Eagle, this formulation provides essential nutrients, vitamins, and amino acids necessary for optimal cell proliferation and maintenance. The MEM AL047S-6x500ML from Himedia is a reliable choice for researchers, students, and educators working in cell biology, virology, and biotechnology.
This ready-to-use liquid medium is formulated to maintain physiological pH and osmolality, ensuring consistent performance in cell culture experiments. Whether you’re culturing primary cells, established cell lines, or conducting viral studies, MEM offers a balanced environment for cellular growth and function.
At eqipped, we understand the importance of quality lab supplies in achieving accurate research outcomes. That’s why we offer authentic Himedia products with proper storage and handling to maintain their efficacy. Each batch of MEM is quality-tested to meet stringent laboratory standards.
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Key Features of MEM Eagle
MEM contains essential components like amino acids, vitamins, inorganic salts, and glucose. The medium is supplemented with sodium bicarbonate as a buffering system. This formulation supports a wide range of cell types including fibroblasts, epithelial cells, and some primary cultures.
The 6x500ml pack size offers convenience for laboratories with moderate to high usage, reducing the need for frequent reordering while maintaining freshness of the medium. The liquid form eliminates preparation time, allowing researchers to focus on their experiments.
Applications in Research
This versatile medium finds applications in various research areas:
- Cell biology studies requiring basic nutrient support
- Viral propagation and plaque assays
- Toxicity testing and drug screening
- Tissue engineering and regenerative medicine research
- Educational demonstrations in cell culture techniques
MEM serves as a base medium that can be further supplemented with serum, growth factors, or antibiotics depending on specific experimental requirements. Its balanced formulation makes it suitable for both adherent and suspension cell cultures.
Storage and Handling
Store MEM at 2-8°C away from direct light. Once opened, use within the recommended period to maintain sterility and nutrient integrity. For best results, warm the medium to 37°C before use and check for any signs of contamination before adding to cell cultures.
Always follow standard aseptic techniques when handling cell culture media to prevent contamination. The medium should be used in a laminar flow hood with proper personal protective equipment.
Why Researchers Choose MEM
MEM remains a popular choice among scientists due to its proven formulation and consistent performance. The medium’s simplicity allows for easy customization while providing all essential nutrients. Its widespread use in published research makes it a reliable standard for comparative studies.
For educational institutions, MEM offers an excellent introduction to cell culture techniques. Students can learn fundamental cell biology concepts using this well-established medium. The 6x500ml packaging is particularly suitable for teaching labs with multiple students.
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Compatibility with Supplements
MEM can be enhanced with various supplements to support specific cell types:
- Fetal bovine serum (FBS) for general cell growth
- L-glutamine for amino acid metabolism
- Non-essential amino acids for specialized cell lines
- Antibiotics like penicillin-streptomycin for contamination control
- Growth factors for specific differentiation studies
The medium’s formulation allows for these additions without significant pH shifts or nutrient imbalances. Researchers should optimize supplement concentrations based on their specific cell lines and experimental conditions.
Quality Assurance
Himedia’s MEM undergoes rigorous quality control testing to ensure batch-to-batch consistency. Each lot is tested for sterility, pH stability, and osmolality. The medium is also screened for endotoxins and mycoplasma contamination to guarantee reliable performance in sensitive applications.
Safety Information
While MEM is generally non-hazardous, standard laboratory safety practices should be followed. Wear appropriate personal protective equipment including gloves and lab coats when handling. In case of contact with eyes or skin, rinse thoroughly with water.
Dispose of used medium according to your institution’s biohazard waste protocols. Unused medium should be autoclaved before disposal to prevent environmental contamination.
FAQs
What is Minimum Essential Medium Eagle (MEM) used for?
Minimum Essential Medium Eagle (MEM) is a widely used cell culture medium designed for the maintenance and growth of mammalian cells in laboratory research. It is commonly used in cell biology, biotechnology, virology, and life science laboratories. :contentReference[oaicite:0]{index=0}
What does HiMedia AL047S contain?
HiMedia AL047S contains Earle’s salts, 2 mM L-glutamine, 1 mM sodium pyruvate, non-essential amino acids (NEAA), and 1.5 g/L sodium bicarbonate, making it suitable for a wide range of mammalian cell culture applications. :contentReference[oaicite:1]{index=1}
How should Minimum Essential Medium Eagle (MEM) be stored?
Store the medium under the manufacturer’s recommended storage conditions. Keep the bottles tightly closed and protected from contamination and prolonged exposure to light to maintain optimal performance. :contentReference[oaicite:2]{index=2}
Is this MEM suitable for research and educational laboratories?
Yes, HiMedia Minimum Essential Medium Eagle (MEM) is suitable for research laboratories, biotechnology facilities, pharmaceutical laboratories, and educational institutions performing mammalian cell culture studies. :contentReference[oaicite:3]{index=3}
Can I buy HiMedia Minimum Essential Medium Eagle (MEM) AL047S online from eqipped?
Yes, eqipped supplies genuine HiMedia cell culture media with secure packaging, reliable delivery across India, and bulk purchasing options for research laboratories, universities, and biotechnology institutions.
