Description
Specifications Table
| Product Name | Moeller Decarboxylase Broth w/ Lysine Hydrochloride |
|---|---|
| Pack Size | 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Laboratory Grade |
| Application | Lysine decarboxylase test, microbiological analysis |
Product Description
Moeller Decarboxylase Broth with Lysine Hydrochloride is a specialized microbiological medium designed to identify bacteria capable of decarboxylating lysine. This broth is widely used in clinical and research laboratories to differentiate enteric bacteria, particularly in the identification of species like Salmonella and Shigella. The medium contains lysine hydrochloride as the substrate, along with a pH indicator that changes color upon decarboxylation, providing clear and reliable results.
When selecting a decarboxylase test medium, accuracy and consistency are critical. This broth ensures optimal performance by maintaining the right balance of nutrients and indicators, making it a trusted choice for microbiologists. Whether you’re conducting routine diagnostic tests or advanced research, this medium delivers dependable outcomes every time.
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How Moeller Decarboxylase Broth Works
The principle behind this medium is straightforward yet effective. Bacteria that possess lysine decarboxylase enzymes convert lysine into cadaverine, an alkaline compound. As the pH rises, the bromocresol purple indicator in the broth shifts from yellow to purple, signaling a positive reaction. This visual change simplifies interpretation, reducing the risk of errors in bacterial identification.
To use, simply inoculate the broth with your bacterial sample and incubate under appropriate conditions. Observe the color change after 24 to 48 hours. A purple hue confirms lysine decarboxylase activity, while a yellow or unchanged color indicates a negative result.
Applications in Microbiology
This broth is indispensable in various microbiological applications, including:
- Identification of enteric pathogens in clinical samples
- Differentiation between Escherichia coli and other coliforms
- Research on bacterial metabolism and enzyme activity
- Quality control in food and pharmaceutical microbiology
Its versatility makes it a staple in labs across India, from educational institutions to advanced research facilities. If you’re looking to buy lysine decarboxylase test medium, this product offers the reliability you need.
Why This Medium Stands Out
Not all decarboxylase test media are created equal. What sets Moeller Decarboxylase Broth apart is its formulation, which minimizes false positives and ensures consistent performance. The inclusion of lysine hydrochloride at optimal concentrations guarantees sensitivity, while the pH indicator provides unambiguous results.
Additionally, the powder form allows for easy preparation and storage, making it convenient for labs with varying workloads. Whether you’re processing a few samples or conducting large-scale testing, this medium adapts to your needs.
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Safety and Handling
While this product is generally safe for laboratory use, standard microbiological precautions should be followed. Always wear appropriate personal protective equipment (PPE) when handling bacterial cultures. Dispose of used media according to local biosafety regulations to prevent contamination.
FAQs
What is Moeller Decarboxylase Broth used for?
It is used to detect lysine decarboxylase activity in bacteria, helping identify species like Salmonella and Shigella.
How do I interpret the results?
A purple color indicates a positive reaction (lysine decarboxylase present), while yellow means negative.
Can I use this broth for other decarboxylase tests?
No, this medium is specifically formulated for lysine decarboxylase testing. For other amino acids, use the appropriate Moeller broth variant.
What is the shelf life of this product?
The powder has a long shelf life when stored in a cool, dry place. Prepared broth should be used within 24 hours.
Where can I buy lysine decarboxylase test medium online?
You can purchase Moeller Decarboxylase Broth from eqipped, ensuring authentic SRL Chem products with reliable delivery.
