Description
Specifications Table
Product Name – MOPS, 99.5%, for molecular biology, Dnase, Rnase, Prot. free, for peptide sequencing
Quantity/Pack Size – 100GR, 500GR
Form – Powder
Grade – Molecular Biology (99.5% purity)
Application – Peptide sequencing, nucleic acid electrophoresis, protein studies
Product Overview
MOPS (3-(N-morpholino)propanesulfonic acid) at 99.5% purity is a zwitterionic buffer designed for precision in molecular biology applications where pH stability is critical. This high-grade reagent is rigorously tested to be free of DNase, RNase, and protease contaminants, ensuring reliable performance in sensitive assays like peptide sequencing, nucleic acid electrophoresis, and protein characterization. Its buffering range (pH 6.5–7.9) makes it ideal for maintaining physiological pH in cell culture media, enzyme reactions, and chromatographic separations. The powder form allows for flexible preparation of solutions at desired concentrations, while its low UV absorbance minimizes interference in spectrophotometric analyses. Compatible with most downstream applications, this MOPS buffer eliminates the risk of nuclease or protease degradation, preserving the integrity of biomolecules. Store under dry conditions to prevent hygroscopicity and extend shelf life.
FAQs
1. What is the buffering range of this MOPS reagent?
This MOPS buffer has an effective buffering range between pH 6.5 and 7.9, making it suitable for applications requiring near-neutral pH conditions.
2. Is this MOPS compatible with cell culture experiments?
Yes, its high purity and absence of enzymatic contaminants make it safe for cell culture media supplementation, provided it is sterilized before use.
3. How should I prepare a 10X MOPS solution for electrophoresis?
Dissolve 41.86 g of MOPS powder in 800 mL of nuclease-free water, adjust pH to 7.0 with NaOH, and bring the volume to 1 L. Sterilize by filtration if required.
4. Can this MOPS be used as an alternative to HEPES in protein studies?
While both are Good’s buffers, MOPS is preferred for applications below pH 8.0, whereas HEPES is better suited for pH 7.2–8.2. Test compatibility with your specific assay.
5. What is the shelf life of unopened MOPS powder?
When stored in a cool, dry place away from moisture, the unopened powder remains stable for up to 3 years from the date of manufacture.
