Description
Specifications Table
Product Name – MOPS, 99%, for biochemistry
Quantity/Pack Size – 25GR, 100GR, 500GR, 2.5KG
Form – White crystalline powder
Grade – 99% (Biochemistry Grade)
Application – pH buffering in molecular biology, protein studies, and cell culture
Product Overview
MOPS (3-(N-morpholino)propanesulfonic acid) is a zwitterionic buffering agent widely recognized for its exceptional performance in biochemical and molecular biology applications. With a purity of 99%, this high-grade MOPS ensures reliable pH stabilization between 6.5 and 7.9, making it indispensable for experiments requiring strict pH control, such as protein electrophoresis, cell culture media preparation, and enzymatic assays. Its non-toxic and non-hygroscopic nature minimizes interference with biological systems, while its high water solubility allows for easy dissolution without residue. The chemical stability of MOPS ensures long-term consistency in experimental conditions, reducing variability in results. Unlike traditional buffers like phosphate or Tris, MOPS does not bind metal ions, which is critical for applications involving metalloenzymes or nucleic acid studies. The powder form allows for flexible preparation of solutions at varying concentrations, catering to diverse laboratory protocols. Whether used in DNA/RNA extraction, chromatography, or microbial growth studies, this biochemistry-grade MOPS delivers reproducibility and precision, meeting the stringent demands of modern research laboratories.
FAQs
1. What is the ideal pH range for MOPS buffer in biochemical applications?
MOPS is most effective as a buffer between pH 6.5 and 7.9, making it suitable for physiological studies and protein-based experiments where neutral pH stability is required. Its pKa of 7.2 aligns closely with intracellular environments, ensuring minimal disruption to biological systems.
2. Can MOPS buffer be autoclaved for sterilization in cell culture applications?
Yes, MOPS buffer solutions can be autoclaved at 121°C for 15–20 minutes without significant degradation. However, it is recommended to adjust the pH post-autoclaving, as slight shifts may occur due to heat exposure. For sensitive applications, filter sterilization using a 0.22 µm membrane is an alternative.
3. How does MOPS compare to HEPES buffer in terms of compatibility with metal ions?
MOPS has a lower affinity for metal ions compared to HEPES, which can chelate divalent cations like Ca²⁺ and Mg²⁺. This makes MOPS a preferred choice for experiments involving metalloenzymes, nucleic acid interactions, or processes where metal ion availability is critical, such as PCR or restriction enzyme digests.
4. What is the shelf life of MOPS powder when stored under standard laboratory conditions?
When stored in a tightly sealed container at room temperature (15–25°C) and protected from moisture and light, MOPS powder retains its stability and purity for at least 3–5 years. Avoid exposure to humidity, as it can lead to clumping without affecting the chemical’s buffering capacity.
5. Are there any known incompatibilities of MOPS with common laboratory reagents?
MOPS is generally compatible with most biochemical reagents but may interact with strong oxidizing agents or reducing agents under extreme conditions. It is not recommended for use with hypochlorite solutions or concentrated acids. Always verify compatibility in pilot tests when combining MOPS with novel reagents or complex media formulations.
