Description
Specifications Table
| Product Name | MOPS Buffer for molecular biology, 99% |
|---|---|
| Pack Size | 25 Gms, 100 Gms, 500 Gms |
| Form | Powder |
| Grade | Molecular Biology Grade |
| Application | DNA/RNA research, protein studies, pH buffering |
Product Description
When precision matters in your molecular biology experiments, the right buffer can make all the difference. MOPS Buffer (3-Morpholinopropane Sulphonic Acid) is a high-purity, zwitterionic compound designed to maintain stable pH levels in biological systems. With a pKa of 7.2 at 25°C, it’s ideal for applications requiring tight pH control, such as DNA/RNA studies, protein electrophoresis, and cell culture media preparation.
This 99% pure MOPS buffer from SRL Chem ensures minimal interference in sensitive assays, making it a trusted choice for researchers and educators. Whether you’re running PCR, gel electrophoresis, or enzyme assays, this buffer provides consistent performance without introducing contaminants.
For labs and institutions in India, eqipped offers this premium-grade MOPS buffer in convenient pack sizes (25g, 100g, 500g) to suit your research needs. Need reliable buffers for your experiments? Shop MOPS buffer online today for fast, pan-India delivery.
Why MOPS Buffer is Essential for Your Lab
MOPS buffer stands out in molecular biology due to its unique properties:
First, its zwitterionic nature means it resists pH shifts even with temperature changes, unlike traditional buffers like Tris. This stability is critical for experiments where slight pH fluctuations can alter results.
Second, MOPS doesn’t chelate metal ions, making it compatible with enzyme assays that require divalent cations like Mg²⁺ or Ca²⁺. This makes it a go-to choice for PCR and restriction digestion protocols.
Finally, its UV transparency ensures it won’t interfere with spectrophotometric measurements, a key advantage for nucleic acid quantification.
Applications in Research and Education
From academic labs to advanced research facilities, MOPS buffer finds use in:
DNA and RNA electrophoresis: Maintains consistent pH for sharp band resolution.
Cell culture media: Provides stable buffering in CO₂-free environments.
Protein purification: Compatible with chromatography and dialysis processes.
Enzyme kinetics: Non-interfering with metal-dependent enzymes.
For students and educators, this buffer simplifies experimental setups while delivering professional-grade results. Upgrade your lab supplies with high-purity MOPS buffer—order online now.
Handling and Storage Guidelines
To maximize shelf life and performance:
Store MOPS buffer in a cool, dry place away from direct sunlight. Once dissolved, solutions are stable for weeks at 4°C.
Always use molecular biology-grade water for preparation to avoid contamination.
Adjust pH with NaOH or HCl as needed, but avoid excessive heating during dissolution.
Risk Note
While MOPS is generally safe, handle with standard lab precautions. Avoid inhalation or direct contact with skin/eyes. Use in a well-ventilated area or under a fume hood when preparing concentrated solutions.
FAQs
What is the ideal pH range for MOPS buffer?
MOPS buffer works best between pH 6.5–7.9, with optimal buffering capacity at pH 7.2.
Can MOPS buffer be autoclaved?
Yes, but autoclaving may slightly alter pH. For critical applications, filter sterilize instead.
How do I prepare a 1M MOPS buffer solution?
Dissolve 209.3g of MOPS in 800ml water, adjust pH with NaOH, then bring to 1L. Store at 4°C.
Is this MOPS buffer compatible with PCR?
Absolutely. Its stability and non-interference with DNA polymerases make it ideal for PCR buffers.
Where can I buy MOPS buffer in India?
eqipped offers lab-grade MOPS buffer with fast shipping across India. Order online for convenience.
