Description
Specifications Table
Product Name – MOPS sodium salt, 98%
Quantity/Pack Size – 1KG, 25GR, 100GR
Form – Powder
Grade – 98%
Application – pH buffering, biochemical assays, protein studies
Product Overview
MOPS sodium salt (3-(N-morpholino)propanesulfonic acid sodium salt) is a high-purity zwitterionic buffer with a pKa of 7.2 at 25°C, making it ideal for maintaining physiological pH in biological systems. This 98% pure grade ensures minimal impurities, providing consistent performance in sensitive applications like protein electrophoresis, cell culture media, and enzymatic reactions. Its non-toxic, non-volatile nature and resistance to metal ion chelation enhance stability in long-term experiments. The powder form allows for easy dissolution in aqueous solutions, while its compatibility with most biological systems reduces the risk of interference. Unlike traditional phosphate buffers, MOPS maintains buffering capacity in the critical 6.5-7.9 pH range without forming precipitates with calcium or magnesium ions, which is particularly valuable in molecular biology protocols. The sodium salt form improves solubility compared to the free acid, enabling precise concentration adjustments for experimental requirements.
FAQs
1. What is the ideal pH range for MOPS sodium salt buffering?
The effective buffering range for MOPS sodium salt is between pH 6.5 and 7.9, with optimal performance around its pKa of 7.2 at 25°C.
2. Can this MOPS salt be used with calcium or magnesium ions?
Yes, MOPS sodium salt does not form insoluble precipitates with calcium or magnesium ions, unlike phosphate buffers, making it suitable for experiments requiring these divalent cations.
3. How should MOPS sodium salt be stored for long-term stability?
Store the powder in a tightly sealed container at room temperature, protected from moisture and direct light to prevent degradation or clumping.
4. Is this grade of MOPS compatible with cell culture applications?
The 98% pure grade is suitable for most cell culture applications, though additional sterile filtration may be required depending on the specific protocol.
5. What alternatives exist if MOPS doesn’t meet my pH requirements?
For lower pH ranges, consider MES (pKa 6.1) or PIPES (pKa 6.8), while HEPES (pKa 7.5) or TAPS (pKa 8.4) work for higher pH applications.
