Description
Specifications Table
| Product Name | MOPS Sodium Salt Buffer extrapure, 99.5% |
|---|---|
| Pack Size | 50 Gms | 100 Gms | 500 Gms |
| Form | Powder |
| Grade | Extrapure |
| Application | Biochemical research, pH buffering, electrophoresis |
Product Description
What is MOPS Sodium Salt Buffer?
MOPS (3-(N-morpholino)propanesulfonic acid) Sodium Salt Buffer is a zwitterionic buffer widely used in biochemical and molecular biology laboratories. Its extrapure grade (99.5% purity) ensures minimal interference in sensitive experiments, making it ideal for applications requiring precise pH control between 6.5 and 7.9.
Why Use MOPS Sodium Salt Buffer in Your Lab?
This buffer is a staple in research labs due to its stability and compatibility with biological systems. Unlike some buffers, MOPS does not chelate metal ions, making it suitable for enzyme assays and cell culture studies. Its sodium salt form enhances solubility, simplifying preparation for experiments.
Key Applications
MOPS Sodium Salt Buffer is commonly used in:
- Electrophoresis (SDS-PAGE, agarose gels)
- Protein purification and chromatography
- Cell culture media formulation
- Enzyme activity assays
- DNA/RNA extraction protocols
How to Prepare MOPS Buffer Solution
To prepare a 1M MOPS buffer solution:
- Dissolve 209.3 g of MOPS Sodium Salt in 800 mL of distilled water.
- Adjust the pH to the desired value (typically 7.0–7.5) using NaOH or HCl.
- Add distilled water to a final volume of 1 L.
- Sterilize by filtration (0.22 µm) if required for cell culture.
Storage and Handling Tips
Store the powder in a tightly sealed container at room temperature. Once prepared, the buffer solution can be stored at 4°C for short-term use or frozen for long-term storage. Avoid repeated freeze-thaw cycles to maintain stability.
Why eqipped for Your Lab Supplies?
At eqipped, we prioritize quality and reliability. Our MOPS Sodium Salt Buffer is sourced from trusted manufacturers like SRL Chem, ensuring consistency in your research. With multiple pack sizes available, you can choose the right quantity for your lab’s needs.
FAQs
What is the shelf life of MOPS Sodium Salt Buffer?
The powder form has a shelf life of 2–3 years when stored properly. Prepared solutions should be used within 6 months.
Can MOPS buffer be autoclaved?
No, autoclaving can degrade MOPS. Sterilize by filtration instead.
Is MOPS Sodium Salt Buffer compatible with metal ions?
Yes, unlike EDTA-based buffers, MOPS does not chelate metal ions, making it ideal for enzyme studies.
What is the ideal pH range for MOPS buffer?
MOPS is effective between pH 6.5 and 7.9, with optimal buffering at pH 7.2.
How does MOPS compare to other buffers like Tris or HEPES?
MOPS is preferred for its minimal interference in biochemical reactions and stability across a wide temperature range.
